دورية أكاديمية
Cell-cycle analysis of fission yeast cells by flow cytometry.
| العنوان: | Cell-cycle analysis of fission yeast cells by flow cytometry. |
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| المؤلفون: | Knutsen JH; Department of Cell Biology, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway., Rein ID, Rothe C, Stokke T, Grallert B, Boye E |
| المصدر: | PloS one [PLoS One] 2011 Feb 28; Vol. 6 (2), pp. e17175. Date of Electronic Publication: 2011 Feb 28. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: Electronic Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: San Francisco, CA : Public Library of Science |
| مواضيع طبية MeSH: | Flow Cytometry*/methods, Cell Cycle/*physiology , Schizosaccharomyces/*cytology, Cell Cycle/genetics ; Cell Cycle Proteins/genetics ; Cell Proliferation ; G1 Phase/genetics ; Light ; Mitosis/genetics ; Mitosis/physiology ; Models, Biological ; Organisms, Genetically Modified ; Scattering, Radiation ; Schizosaccharomyces/growth & development ; Schizosaccharomyces/physiology ; Schizosaccharomyces pombe Proteins/genetics ; Transcription Factors/genetics ; ras-GRF1/genetics |
| مستخلص: | The cell cycle of the fission yeast, Schizosaccharomyces pombe, does not easily lend itself to analysis by flow cytometry, mainly because cells in G(1) and G(2) phase contain the same amount of DNA. This occurs because fission yeast cells under standard growth conditions do not complete cytokinesis until after G(1) phase. We have devised a flow cytometric method exploiting the fact that cells in G(1) phase contain two nuclei, whereas cells in G(2) are mononuclear. Measurements of the width as well as the total area of the DNA-associated fluorescence signal allows the discrimination between cells in G(1) and in G(2) phase and the cell-cycle progression of fission yeast can be followed in detail by flow cytometry. Furthermore, we show how this method can be used to monitor the timing of cell entry into anaphase. Fission yeast cells tend to form multimers, which represents another problem of flow cytometry-based cell-cycle analysis. Here we present a method employing light-scatter measurements to enable the exclusion of cell doublets, thereby further improving the analysis of fission yeast cells by flow cytometry. |
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| المشرفين على المادة: | 0 (Cell Cycle Proteins) 0 (Schizosaccharomyces pombe Proteins) 0 (Transcription Factors) 0 (cdc10 protein, S pombe) 0 (cdc22 protein, S pombe) 0 (ras-GRF1) |
| تواريخ الأحداث: | Date Created: 20110310 Date Completed: 20110901 Latest Revision: 20240313 |
| رمز التحديث: | 20240313 |
| مُعرف محوري في PubMed: | PMC3046126 |
| DOI: | 10.1371/journal.pone.0017175 |
| PMID: | 21386895 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1932-6203 |
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| DOI: | 10.1371/journal.pone.0017175 |