التفاصيل البيبلوغرافية
| العنوان: |
Detection and Immobilization of Proteins Containing the 6xHis Tag. |
| المؤلفون: |
Kneusel RE; Qiagen, Hilden, Germany., Wulbeck M, Ribbe J |
| المصدر: |
Methods in molecular medicine [Methods Mol Med] 1998; Vol. 13, pp. 309-22. |
| نوع المنشور: |
Journal Article |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: Humana Press Country of Publication: United States NLM ID: 101123138 Publication Model: Print Cited Medium: Print ISSN: 1543-1894 (Print) Linking ISSN: 15431894 NLM ISO Abbreviation: Methods Mol Med Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: |
Original Publication: Totowa, N.J. : Humana Press, c1996- |
| مستخلص: |
Many systems have been developed for the heterologous expression of recombinant proteins. They are often based on the fusion of the protein of interest with a naturally occurring protein (glutathione S-transferase [1], maltose binding protein [2], or protein A [3]) and using their natural affinity to substrates (glutathione, amylose, or immunoglobulins) coupled to columns in the puriticat1on step. Problems with these systems are that the affinity tag may affect protein structure and function, and that proteins in insoluble fractions cannot be adequately purified. |
| تواريخ الأحداث: |
Date Created: 20110311 Date Completed: 20121002 Latest Revision: 20110310 |
| رمز التحديث: |
20221213 |
| DOI: |
10.1385/0-89603-485-2:309 |
| PMID: |
21390851 |
| قاعدة البيانات: |
MEDLINE |
