التفاصيل البيبلوغرافية
| العنوان: |
[Investigation of exosomes secreted by different normal and malignant cells in vitro and in vivo]. |
| المؤلفون: |
Filatov MV, Landa SB, Pantina RA, Garmaĭ IuP |
| المصدر: |
Klinicheskaia laboratornaia diagnostika [Klin Lab Diagn] 2010 Dec (12), pp. 35-43. |
| نوع المنشور: |
Journal Article |
| اللغة: |
Russian |
| بيانات الدورية: |
Publisher: Meditsina Country of Publication: Russia (Federation) NLM ID: 9432021 Publication Model: Print Cited Medium: Print ISSN: 0869-2084 (Print) Linking ISSN: 08692084 NLM ISO Abbreviation: Klin Lab Diagn Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Moskva : "Meditsina", 1992- |
| مواضيع طبية MeSH: |
Dendritic Cells*/metabolism , Dendritic Cells*/ultrastructure , Exosomes*/metabolism , Exosomes*/ultrastructure , Fibroblasts*/metabolism , Fibroblasts*/ultrastructure , Neoplasms*/metabolism , Neoplasms*/ultrastructure, HeLa Cells ; Histocompatibility Antigens Class I/metabolism ; Humans ; Microscopy, Atomic Force/methods |
| مستخلص: |
Laser correlation spectroscopy, atomic force microscopy, and immunoaffinity chromatography were used to characterize exosomes produced by different human cells. The exosomes secreted into a culture medium by normal fibroblasts, dendritic cells, lymphocytes, as well as malignant cells obtained from tumors of various tissue origins. The similar investigations were made for exosomes detectable in plasma and cerebrospinal fluid. The dynamic light scattering technique has demonstrated that the exosomes from different sources are homogenous and similar in size of the order of 20 and 90 nm. The exceptional homogeneity of exosomes was confirmed by atomic force microscopy. The immunoaffinity method has shown that all the exosomes under study carry antigenic determinants recognizable by antibodies to the major histocompatibility complex of type 1 (HLA-ABC). A method is proposed for evidence-based detection of exosomes in various biological fluids. For this, dynamic light scattering detects 20- and 90-nm particles and whether they can be removed by immunoaffinity chromatography with HLA-ABC antibodies is checked. |
| المشرفين على المادة: |
0 (Histocompatibility Antigens Class I) |
| تواريخ الأحداث: |
Date Created: 20110315 Date Completed: 20110406 Latest Revision: 20190816 |
| رمز التحديث: |
20231215 |
| PMID: |
21395053 |
| قاعدة البيانات: |
MEDLINE |
