دورية أكاديمية
Chromatin-associated protein kinase C-θ regulates an inducible gene expression program and microRNAs in human T lymphocytes.
| العنوان: | Chromatin-associated protein kinase C-θ regulates an inducible gene expression program and microRNAs in human T lymphocytes. |
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| المؤلفون: | Sutcliffe EL; Department of Immunology, John Curtin School of Medical Research, Australian National University, Canberra, ACT 2601, Australia., Bunting KL, He YQ, Li J, Phetsouphanh C, Seddiki N, Zafar A, Hindmarsh EJ, Parish CR, Kelleher AD, McInnes RL, Taya T, Milburn PJ, Rao S |
| المصدر: | Molecular cell [Mol Cell] 2011 Mar 18; Vol. 41 (6), pp. 704-19. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 9802571 Publication Model: Print Cited Medium: Internet ISSN: 1097-4164 (Electronic) Linking ISSN: 10972765 NLM ISO Abbreviation: Mol Cell Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge Ma : Cell Press Original Publication: Cambridge, Mass. : Cell Press, c1997- |
| مواضيع طبية MeSH: | Gene Expression Regulation*, Chromatin/*metabolism , Isoenzymes/*metabolism , MicroRNAs/*metabolism , Protein Kinase C/*metabolism , T-Lymphocytes/*physiology, Cell Line, Tumor ; Cell Nucleus/metabolism ; Humans ; Interleukin-2/genetics ; Isoenzymes/genetics ; Jurkat Cells ; MicroRNAs/genetics ; Promoter Regions, Genetic ; Protein Kinase C/genetics ; Protein Kinase C-theta ; RNA Polymerase II/genetics ; RNA Polymerase II/metabolism ; Ribosomal Protein S6 Kinases, 90-kDa/genetics ; Ribosomal Protein S6 Kinases, 90-kDa/metabolism ; T-Lymphocytes/cytology ; Transcription, Genetic |
| مستخلص: | Studies in yeast demonstrate that signaling kinases have a surprisingly active role in the nucleus, where they tether to chromatin and modulate gene expression programs. Despite these seminal studies, the nuclear mechanism of how signaling kinases control transcription of mammalian genes is in its infancy. Here, we provide evidence for a hitherto unknown function of protein kinase C-theta (PKC-θ), which physically associates with the regulatory regions of inducible immune response genes in human T cells. Chromatin-anchored PKC-θ forms an active nuclear complex by interacting with RNA polymerase II, the histone kinase MSK-1, and the adaptor molecule 14-3-3ζ. ChIP-on-chip reveals that PKC-θ binds to promoters and transcribed regions of genes, as well as to microRNA promoters that are crucial for cytokine regulation. Our results provide a molecular explanation for the role of PKC-θ not only in normal T cell function, but also in circumstances of its ectopic expression in cancer. (Copyright © 2011 Elsevier Inc. All rights reserved.) |
| المشرفين على المادة: | 0 (Chromatin) 0 (Interleukin-2) 0 (Isoenzymes) 0 (MicroRNAs) EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 90-kDa) EC 2.7.11.1 (mitogen and stress-activated protein kinase 1) EC 2.7.11.13 (PRKCQ protein, human) EC 2.7.11.13 (Protein Kinase C) EC 2.7.11.13 (Protein Kinase C-theta) EC 2.7.7.- (RNA Polymerase II) |
| تواريخ الأحداث: | Date Created: 20110323 Date Completed: 20110519 Latest Revision: 20171116 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.molcel.2011.02.030 |
| PMID: | 21419345 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1097-4164 |
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| DOI: | 10.1016/j.molcel.2011.02.030 |