دورية أكاديمية
Universal noninvasive detection of solid organ transplant rejection.
| العنوان: | Universal noninvasive detection of solid organ transplant rejection. |
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| المؤلفون: | Snyder TM; Department of Applied Physics, Stanford University, Stanford, The Howard Hughes Medical Institute, CA 94305, USA., Khush KK, Valantine HA, Quake SR |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2011 Apr 12; Vol. 108 (15), pp. 6229-34. Date of Electronic Publication: 2011 Mar 28. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : National Academy of Sciences |
| مواضيع طبية MeSH: | Heart Transplantation*, DNA/*blood , Graft Rejection/*diagnosis , High-Throughput Nucleotide Sequencing/*methods , Monitoring, Physiologic/*methods, Chromosomes, Human, Y/genetics ; DNA/genetics ; Graft Rejection/blood ; Graft Rejection/pathology ; Humans ; Polymorphism, Single Nucleotide ; Tissue Donors |
| مستخلص: | It is challenging to monitor the health of transplanted organs, particularly with respect to rejection by the host immune system. Because transplanted organs have genomes that are distinct from the recipient's genome, we used high throughput shotgun sequencing to develop a universal noninvasive approach to monitoring organ health. We analyzed cell-free DNA circulating in the blood of heart transplant recipients and observed significantly increased levels of cell-free DNA from the donor genome at times when an endomyocardial biopsy independently established the presence of acute cellular rejection in these heart transplant recipients. Our results demonstrate that cell-free DNA can be used to detect an organ-specific signature that correlates with rejection, and this measurement can be made on any combination of donor and recipient. This noninvasive test holds promise for replacing the endomyocardial biopsy in heart transplant recipients and may be applicable to other solid organ transplants. |
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| معلومات مُعتمدة: | United States Howard Hughes Medical Institute |
| المشرفين على المادة: | 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 20110330 Date Completed: 20110627 Latest Revision: 20220409 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC3076856 |
| DOI: | 10.1073/pnas.1013924108 |
| PMID: | 21444804 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1091-6490 |
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| DOI: | 10.1073/pnas.1013924108 |