دورية أكاديمية
[The effect of low temperatures on the viability of human epidermal keratinocytes found at different stages of differentiation].
| العنوان: | [The effect of low temperatures on the viability of human epidermal keratinocytes found at different stages of differentiation]. |
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| المؤلفون: | Raĭdan M, Shubin NA, Blinova MI, Prokhorov GG, Pinaev GP |
| المصدر: | Tsitologiia [Tsitologiia] 2011; Vol. 53 (1), pp. 22-30. |
| نوع المنشور: | Journal Article |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Rossiiskaia Akademiia Nauk Country of Publication: Russia (Federation) NLM ID: 0417363 Publication Model: Print Cited Medium: Print ISSN: 0041-3771 (Print) Linking ISSN: 00413771 NLM ISO Abbreviation: Tsitologiia Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Moskva : Rossiiskaia Akademiia Nauk Original Publication: Moskva, Akademiia nauk SSSR. |
| مواضيع طبية MeSH: | Cold Temperature* , Epidermal Cells*, Keratinocytes/*cytology, Cell Count ; Cell Culture Techniques ; Cell Differentiation/physiology ; Cell Proliferation ; Cell Survival ; Cells, Cultured ; Cervicoplasty ; Cryotherapy ; Freezing ; Humans ; Microscopy, Fluorescence ; Statistical Distributions ; Stem Cells |
| مستخلص: | The aim of this study was a comparative analysis to the degree of stability of human epidermal cells found at different stages of differentiation to low temperatures. The effect of different subzero temperatures of liquid nitrogen vapor on keratinocytes found both in human skin fragments and as isolated cells extracted from skin fragments has been studied. The degree of stability of epidermal cells low temperatures was evaluated by their ability to form a multilayer stratum in culture; hence this phenomenon explains the survival of a sufficient amount of proliferative cells after exposure to subzero temperatures. Quantitative analysis of the ratio of epidermal stem, transitory and differentiated cells in a population of viable cells before and after exposure to low temperatures were determined using antibodies corresponding to their different stages of differentiation. The results of this research show that the stability of human epidermal cells to low temperature differs depending on their stage of differentiation both in situ and in vitro. Epidermal stem cells and transitory cells are more stable than differentiated cells. |
| تواريخ الأحداث: | Date Created: 20110409 Date Completed: 20110701 Latest Revision: 20181201 |
| رمز التحديث: | 20240628 |
| PMID: | 21473115 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0041-3771 |
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