دورية أكاديمية
[Genetic bases of methionine dependence in Yersinia pestis strains of major and non-major subspecies].
| العنوان: | [Genetic bases of methionine dependence in Yersinia pestis strains of major and non-major subspecies]. |
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| المؤلفون: | Odinokov GN, Eroshenko GA, Krasnov IaM, Kukleva LM, Shavina NIu, Pavlova AI, Kutyrev VV |
| المصدر: | Genetika [Genetika] 2011 Mar; Vol. 47 (3), pp. 332-8. |
| نوع المنشور: | Journal Article |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Izdatelstvo Nauka Country of Publication: Russia (Federation) NLM ID: 0047354 Publication Model: Print Cited Medium: Print ISSN: 0016-6758 (Print) Linking ISSN: 00166758 NLM ISO Abbreviation: Genetika Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Moskva : Izdatelstvo Nauka |
| مواضيع طبية MeSH: | Methionine/*genetics , Methionine/*metabolism , Yersinia pestis/*genetics , Yersinia pestis/*metabolism, Carbon-Oxygen Lyases/genetics ; Carbon-Oxygen Lyases/metabolism ; Sequence Deletion |
| مستخلص: | Structural and functional organization of genes responsible for biosynthesis of amino acid methionine, which plays a leading role in cellular metabolism of bacteria, was studied in 24 natural Yersinia pestis strains of the major and minor subspecies from various natural plague foci located in the territory of Russian Federation and neighbouring foreign countries, and also in Y. pestis and Y. pseudotuberculosis strains recorded in the files of NCBI GenBank database. Conservatism of genes metA, metB, metC, metE, and metH as well as regulatory genes metR and metJ involved in biosynthesis of this amino acid was established. Sequencing of the variable locus of gene metB in natural Y. pestis strains of major and minor subspecies revealed that the reason for the methionine dependence of strains belonging to the major subspecies is a deletion of a single nucleotide (-G) in the 988 position from the beginning of the gene, whereas this dependence in strains belonging to subspecies hissarica results from the appearance of a single nucleotide (+G) insertion in the 989 position of gene metB. These mutations are absent in strains of the caucasica, altaica, and ulegeica subspecies of the plague agent and in strains of pseudotuberculosis microbe, which correlates with their capacity for methionine biosynthesis. |
| المشرفين على المادة: | AE28F7PNPL (Methionine) EC 2.5.1.48 (O-succinylhomoserine (thiol)-lyase) EC 4.2.- (Carbon-Oxygen Lyases) |
| تواريخ الأحداث: | Date Created: 20110506 Date Completed: 20110608 Latest Revision: 20201209 |
| رمز التحديث: | 20240628 |
| PMID: | 21542303 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0016-6758 |
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