دورية أكاديمية
RNA versus DNA (NucliSENS EasyQ HIV-1 v1.2 versus Amplicor HIV-1 DNA test v1.5) for early diagnosis of HIV-1 infection in infants in Senegal.
| العنوان: | RNA versus DNA (NucliSENS EasyQ HIV-1 v1.2 versus Amplicor HIV-1 DNA test v1.5) for early diagnosis of HIV-1 infection in infants in Senegal. |
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| المؤلفون: | Kébé K; Laboratoire de Bactériologie Virologie, Hôpital Aristide le Dantec, Université Cheikh Anta Diop, Dakar, Senegal., Ndiaye O, Ndiaye HD, Mengue PM, Guindo PM, Diallo S, Léye N, Gueye SB, Diallo AG, Kane CT, Mboup S |
| المصدر: | Journal of clinical microbiology [J Clin Microbiol] 2011 Jul; Vol. 49 (7), pp. 2590-3. Date of Electronic Publication: 2011 May 04. |
| نوع المنشور: | Comparative Study; Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7505564 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-660X (Electronic) Linking ISSN: 00951137 NLM ISO Abbreviation: J Clin Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Society for Microbiology. |
| مواضيع طبية MeSH: | Reagent Kits, Diagnostic*, DNA, Viral/*isolation & purification , HIV Infections/*diagnosis , HIV-1/*isolation & purification , Molecular Diagnostic Techniques/*methods , RNA, Viral/*isolation & purification , Virology/*methods, DNA, Viral/blood ; DNA, Viral/genetics ; Desiccation ; Early Diagnosis ; HIV-1/genetics ; Humans ; Infant ; Infant, Newborn ; RNA, Viral/blood ; RNA, Viral/genetics ; Senegal ; Sensitivity and Specificity ; Specimen Handling/methods |
| مستخلص: | The objective of this study was to compare the performance of the NucliSENS EasyQ HIV-1 v1.2 platform (bioMérieux, France) to the Amplicor HIV-1 DNA test v1.5 (Roche Molecular Systems, Switzerland) in detecting HIV-1 infection in infants using venipuncture-derived whole blood in tubes and dried blood spots. A total of 149 dried blood spots and 43 EDTA-anticoagulated peripheral blood samples were collected throughout Dakar and other areas in Senegal from infants and children aged 3 weeks to 24 months who were born to HIV-1-infected mothers. Samples were tested using the NucliSENS and Amplicor technologies. The NucliSENS and Amplicor results were 100% concordant using either EDTA-anticoagulated peripheral blood or dried blood spots. Compared to Amplicor, the sensitivity and specificity of the NucliSENS test were 100%. The NucliSENS EasyQ HIV-1 RNA assay performed as well as the Amplicor HIV-1 DNA test in detecting HIV-1 infection in infants. In addition, this platform can give an indication of the viral load baseline. The NucliSENS EasyQ platform is a good alternative for early infant diagnosis of HIV-1 infection. |
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| المشرفين على المادة: | 0 (DNA, Viral) 0 (RNA, Viral) 0 (Reagent Kits, Diagnostic) |
| تواريخ الأحداث: | Date Created: 20110506 Date Completed: 20110926 Latest Revision: 20211020 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC3147881 |
| DOI: | 10.1128/JCM.02402-10 |
| PMID: | 21543563 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-660X |
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| DOI: | 10.1128/JCM.02402-10 |