دورية أكاديمية
Recursive construction and error correction of DNA molecules and libraries from synthetic and natural DNA.
| العنوان: | Recursive construction and error correction of DNA molecules and libraries from synthetic and natural DNA. |
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| المؤلفون: | Yehezkel TB; Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel., Linshiz G, Kaplan S, Gronau I, Ravid S, Adar R, Shapiro E |
| المصدر: | Methods in enzymology [Methods Enzymol] 2011; Vol. 498, pp. 207-45. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0212271 Publication Model: Print Cited Medium: Internet ISSN: 1557-7988 (Electronic) Linking ISSN: 00766879 NLM ISO Abbreviation: Methods Enzymol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Gene Library* , Genes, Synthetic*, DNA/*genetics , Synthetic Biology/*methods, Algorithms ; Base Sequence ; Computational Biology/methods ; DNA/biosynthesis ; Electrophoresis, Capillary/methods ; Genetic Engineering/methods ; Molecular Sequence Data ; Oligonucleotides/genetics ; Polymerase Chain Reaction/methods ; Proteins/chemistry ; Proteins/genetics |
| مستخلص: | Making error-free, custom DNA assemblies from potentially faulty building blocks is a fundamental challenge in synthetic biology. Here, we show how recursion can be used to address this challenge using a recursive procedure that constructs error-free DNA molecules and their libraries from error-prone synthetic oligonucleotides and naturally existing DNA. Specifically, we describe how divide and conquer (D&C), the quintessential recursive problem-solving technique, is applied in silico to divide target DNA sequences into overlapping, albeit error prone, oligonucleotides, and how recursive construction is applied in vitro to combine them to form error-prone DNA molecules. To correct DNA sequence errors, error-free fragments of these molecules are then identified, extracted, and used as new, typically longer and more accurate, inputs to another iteration of the recursive construction procedure; the entire process repeats until an error-free target molecule is formed. The method allows combining synthetic and natural DNA fragments into error-free designer DNA libraries, thus providing a foundation for the design and construction of complex synthetic DNA assemblies. (Copyright © 2011 Elsevier Inc. All rights reserved.) |
| المشرفين على المادة: | 0 (Oligonucleotides) 0 (Proteins) 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 20110524 Date Completed: 20110906 Latest Revision: 20110523 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/B978-0-12-385120-8.00010-3 |
| PMID: | 21601680 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1557-7988 |
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| DOI: | 10.1016/B978-0-12-385120-8.00010-3 |