دورية أكاديمية
[Sialic acids and O-acetyl groups as markers of biological activity of microbial polysaccharides in plague and cholera agents].
| العنوان: | [Sialic acids and O-acetyl groups as markers of biological activity of microbial polysaccharides in plague and cholera agents]. |
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| المؤلفون: | Gromova OV, Pavlova VI, Taranenko TM, Guseva NP, Kireev MN, Khramchenkova TA |
| المصدر: | Zhurnal mikrobiologii, epidemiologii i immunobiologii [Zh Mikrobiol Epidemiol Immunobiol] 2011 Mar-Apr (2), pp. 3-7. |
| نوع المنشور: | Journal Article |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: FBUN T︠S︡NII Ėpidemiologii Rospotrebnadzora Country of Publication: Russia (Federation) NLM ID: 0415217 Publication Model: Print Cited Medium: Print ISSN: 0372-9311 (Print) Linking ISSN: 03729311 NLM ISO Abbreviation: Zh Mikrobiol Epidemiol Immunobiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2018, no. 4 (ii︠u︡lʹ-avgust)- >: Moskva : FBUN T︠S︡NII Ėpidemiologii Rospotrebnadzora Original Publication: Moskva : Izd-vo "Medit︠s︡ina" |
| مواضيع طبية MeSH: | Vibrio cholerae*/chemistry , Vibrio cholerae*/immunology , Yersinia pestis*/chemistry , Yersinia pestis*/immunology, Sialic Acids/*analysis, Allergens/analysis ; Allergens/isolation & purification ; Animals ; Bacterial Proteins/analysis ; Bacterial Proteins/isolation & purification ; Biomarkers/analysis ; Cell Culture Techniques ; Cholera/immunology ; Cholera/pathology ; Humans ; Immunodiffusion ; O Antigens/analysis ; O Antigens/isolation & purification ; Plague/immunology ; Plague/pathology ; Plasminogen Activators/analysis ; Plasminogen Activators/isolation & purification ; Rodentia ; Serologic Tests ; Sialic Acids/immunology ; Structure-Activity Relationship |
| مستخلص: | Aim: To determine sialic acids and O-acetyl groups content in Yersinia pestis and Vibrio cholerae antigens in order to establish their association with biological activity. Materials and Methods: The following antigens of Y. pestis EV NIIEG strain--capsular antigen (F1), major somatic antigen (MSA), lipopolysaccharide (LPS), Pla-protease, allergen pestin PP--as well as O-antigens (O-AG) of V. cholerae serogroups O1 and O139 were used in the study. Sialic acids were identified by the thiobarbituric method, and O-acetyl groups--according to Alicino. Specific polysaccharides in the MSA and O-antigens were detected by the immunodiffusion assay. Results: Sialic acids were found in LPS, Pla-protease, allergen pestin PP, and all cholera O-AG; their absence was demonstrated in MSA and F1. O-acetyl groups were identified in cholera O-AG of both studied serogroups as well as in LPS, Pla-protease, MSA and pestin PP of Y. pestis. Tendency to correlation between O-acetyl groups content in MSA and serological activity titer was observed. Conclusion: Sialic acids and O-acetyl groups identified in carbohydrate-containing antigens of Y. pestis and V. cholerae could be characterized as reaction-active markers of pathogenetic mechanisms of cholera and plague infections as well as immunochemical activity of microbial polysaccharides. |
| المشرفين على المادة: | 0 (Allergens) 0 (Bacterial Proteins) 0 (Biomarkers) 0 (O Antigens) 0 (Sialic Acids) 0 (caf1 protein, Yersinia pestis) EC 3.4.21.- (Pla protease, Yersinia pestis) EC 3.4.21.- (Plasminogen Activators) |
| تواريخ الأحداث: | Date Created: 20110524 Date Completed: 20110701 Latest Revision: 20201209 |
| رمز التحديث: | 20240628 |
| PMID: | 21604388 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0372-9311 |
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