دورية أكاديمية
Two binding sites for the herpes simplex virus type 1 UL9 protein are required for efficient activity of the oriS replication origin.
| العنوان: | Two binding sites for the herpes simplex virus type 1 UL9 protein are required for efficient activity of the oriS replication origin. |
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| المؤلفون: | Weir HM; Medical Research Council Virology Unit, Institute of Virology, Glasgow, U.K., Stow ND |
| المصدر: | The Journal of general virology [J Gen Virol] 1990 Jun; Vol. 71 ( Pt 6), pp. 1379-85. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 0077340 Publication Model: Print Cited Medium: Print ISSN: 0022-1317 (Print) Linking ISSN: 00221317 NLM ISO Abbreviation: J Gen Virol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : London : Microbiology Society Original Publication: London, Cambridge Univ. Press for the Society for General Microbiology. |
| مواضيع طبية MeSH: | DNA Replication*, DNA, Viral/*metabolism , DNA-Binding Proteins/*metabolism , Simplexvirus/*genetics , Viral Proteins/*metabolism, Base Sequence ; Binding Sites ; DNA-Binding Proteins/genetics ; Molecular Sequence Data ; Mutation ; Oligodeoxyribonucleotides/metabolism ; Simplexvirus/metabolism ; Viral Proteins/genetics ; Virus Replication |
| مستخلص: | Two sites within the short region origin of DNA replication (oriS) in herpes simplex virus type 1 (HSV-1) which bind the product of the UL9 gene have previously been identified. One of these sites (site I) contains an 11 bp sequence which is also present in oriS of varicella-zoster virus, and the other (site II) includes a related element differing in two positions. A third sequence (motif III), which lies close to binding site I, differs from the site I element at only a single position. We have deleted specifically each of these three 11 bp sequences from within functional copies of HSV-1 oriS and have examined the effects on origin activity and binding of the UL9 protein. Gel retardation analyses confirmed the important roles of the regions deleted from sites I and II in interacting with the UL9 protein. In transient replication assays, copies of oriS lacking the site I or II elements exhibited undetectable or residual (4 to 8%) activity respectively. The UL9 protein did not bind to motif III even in the absence of site I sequences, although removal of the motif III sequence caused a small reduction in oriS activity. A single base change which converted the sequence within binding site I to that of motif III was sufficient to abolish both the interaction of the UL9 gene product at this locus and the replicative ability of oriS. Therefore, interaction of the UL9 protein with binding site I is essential for origin activity, but the presence of binding site II is also required for efficient replication. |
| المشرفين على المادة: | 0 (DNA, Viral) 0 (DNA-Binding Proteins) 0 (Oligodeoxyribonucleotides) 0 (Viral Proteins) 115004-77-8 (UL9 protein, Human herpesvirus 1) |
| تواريخ الأحداث: | Date Created: 19900601 Date Completed: 19900719 Latest Revision: 20061115 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1099/0022-1317-71-6-1379 |
| PMID: | 2161905 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0022-1317 |
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| DOI: | 10.1099/0022-1317-71-6-1379 |