دورية أكاديمية
أعرض في EDS

Roles of DNA polymerase I in leading and lagging-strand replication defined by a high-resolution mutation footprint of ColE1 plasmid replication.

التفاصيل البيبلوغرافية
العنوان: Roles of DNA polymerase I in leading and lagging-strand replication defined by a high-resolution mutation footprint of ColE1 plasmid replication.
المؤلفون: Allen JM; Department of Microbiology and Environmental Toxicology, University of California Santa Cruz, 1156 High Street Santa Cruz, CA 95060, USA., Simcha DM, Ericson NG, Alexander DL, Marquette JT, Van Biber BP, Troll CJ, Karchin R, Bielas JH, Loeb LA, Camps M
المصدر: Nucleic acids research [Nucleic Acids Res] 2011 Sep 01; Vol. 39 (16), pp. 7020-33. Date of Electronic Publication: 2011 May 26.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.
مواضيع طبية MeSH: DNA Replication* , Mutation*, DNA Polymerase I/*metabolism , Plasmids/*biosynthesis, Base Sequence ; DNA/metabolism ; DNA Footprinting ; DNA Polymerase I/genetics ; DNA Polymerase I/physiology ; Databases, Nucleic Acid ; Plasmids/chemistry
مستخلص: DNA polymerase I (pol I) processes RNA primers during lagging-strand synthesis and fills small gaps during DNA repair reactions. However, it is unclear how pol I and pol III work together during replication and repair or how extensive pol I processing of Okazaki fragments is in vivo. Here, we address these questions by analyzing pol I mutations generated through error-prone replication of ColE1 plasmids. The data were obtained by direct sequencing, allowing an accurate determination of the mutation spectrum and distribution. Pol I's mutational footprint suggests: (i) during leading-strand replication pol I is gradually replaced by pol III over at least 1.3 kb; (ii) pol I processing of Okazaki fragments is limited to ∼20 nt and (iii) the size of Okazaki fragments is short (∼250 nt). While based on ColE1 plasmid replication, our findings are likely relevant to other pol I replicative processes such as chromosomal replication and DNA repair, which differ from ColE1 replication mostly at the recruitment steps. This mutation footprinting approach should help establish the role of other prokaryotic or eukaryotic polymerases in vivo, and provides a tool to investigate how sequence topology, DNA damage, or interactions with protein partners may affect the function of individual DNA polymerases.
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معلومات مُعتمدة: CA102029 United States CA NCI NIH HHS; CA115802 United States CA NCI NIH HHS; CA116429-04 United States CA NCI NIH HHS
المشرفين على المادة: 0 (Okazaki fragments)
9007-49-2 (DNA)
EC 2.7.7.7 (DNA Polymerase I)
تواريخ الأحداث: Date Created: 20110531 Date Completed: 20111122 Latest Revision: 20211020
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC3167613
DOI: 10.1093/nar/gkr157
PMID: 21622658
قاعدة البيانات: MEDLINE
الوصف
تدمد:1362-4962
DOI:10.1093/nar/gkr157