دورية أكاديمية
A novel method for cryopreservation of individual human spermatozoa.
| العنوان: | A novel method for cryopreservation of individual human spermatozoa. |
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| المؤلفون: | Peng QP; Department of Assisted Reproduction, Shanghai 9th People's Hospital, School of Medicine, Shanghai Jiao Tong University, 639 ZhiZaoJu Road, 200011, Shanghai, China. pengqiuping@hotmail.com, Cao SF, Lyu QF, Xue SG, Jin W, Liu XY, Zhang WJ, Nielsen HI, Kuang YP |
| المصدر: | In vitro cellular & developmental biology. Animal [In Vitro Cell Dev Biol Anim] 2011 Sep; Vol. 47 (8), pp. 565-72. Date of Electronic Publication: 2011 Jun 03. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Germany NLM ID: 9418515 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1543-706X (Electronic) Linking ISSN: 10712690 NLM ISO Abbreviation: In Vitro Cell Dev Biol Anim Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Berlin : Springer Original Publication: Columbia, MD : Tissue Culture Association, c1993- |
| مواضيع طبية MeSH: | Cryopreservation/*methods , Fertilization/*physiology , Semen Preservation/*methods , Sperm Motility/*physiology, Cryoprotective Agents/chemistry ; Equipment Design ; Female ; Humans ; Male ; Oocytes/cytology ; Sperm Injections, Intracytoplasmic/methods ; Spermatozoa/cytology |
| مستخلص: | The purpose of this study is to develop a novel method for the cryopreservation and efficient post-thaw recovery of individual or small numbers of human spermatozoa. Spermatozoa equilibrated in cryoprotectant buffer were injected with an intracytoplasmic sperm injection (ICSI) needle into a droplet of cryoprotectant on a homemade cryoleaf. The droplet was of cryoprotectant and seminal plasma at a ratio of 1:1. The sperm-loaded cryoleaf was slowly lowered over and stored in liquid nitrogen. Spermatozoa were thawed in a 37°C oil bath without dilution and centrifugation. To test the fertilizing ability of these spermatozoa, the recovered spermatozoa were injected by ICSI into 1-d-old or in vitro-matured human oocytes. Fresh spermatozoa from the same semen samples served as controls. The trials were performed in two separate experiments. In the first set of experiments, 92 spermatozoa were thawed and carefully investigated. The spermatozoa from percutaneous epididymal sperm aspiration had a motility recovery of 92.9% (13/14); ejaculated spermatozoa had a motility recovery of 61.5% (48/78), and only 1.3% (1/78) was lost. Together in the first and second set of experiments, the fertilization rates for the fresh and frozen-thawed spermatozoa were 67.6% (25/37) and 60.6% (40/66), respectively (P = 0.052). The mean embryo cleavage rates in the fresh and frozen-thawed groups were 88% (22/25) and 85% (34/40), respectively (P = 0.990). This cryopreservation method for individual or small numbers of human spermatozoa was efficient and simple. These findings make this method a promising technique for the clinical application of ejaculated sperm from oligozoospermic patients. |
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| المشرفين على المادة: | 0 (Cryoprotective Agents) |
| تواريخ الأحداث: | Date Created: 20110604 Date Completed: 20120118 Latest Revision: 20211020 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1007/s11626-011-9428-1 |
| PMID: | 21638160 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1543-706X |
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| DOI: | 10.1007/s11626-011-9428-1 |