دورية أكاديمية
Differential mechanisms of x-ray-induced cell death in human endothelial progenitor cells isolated from cord blood and adults.
| العنوان: | Differential mechanisms of x-ray-induced cell death in human endothelial progenitor cells isolated from cord blood and adults. |
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| المؤلفون: | Mendonca MS; Department of Radiation Oncology, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA. mmendonc@iupui.edu, Chin-Sinex H, Dhaemers R, Mead LE, Yoder MC, Ingram DA |
| المصدر: | Radiation research [Radiat Res] 2011 Aug; Vol. 176 (2), pp. 208-16. Date of Electronic Publication: 2011 Jun 10. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Radiation Research Society Country of Publication: United States NLM ID: 0401245 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1938-5404 (Electronic) Linking ISSN: 00337587 NLM ISO Abbreviation: Radiat Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Bozeman, MT : Radiation Research Society Original Publication: Charlottesville, VA : Kluge Carden Jennnings Pub. Co. |
| مواضيع طبية MeSH: | Adult Stem Cells/*cytology , Adult Stem Cells/*radiation effects , Apoptosis/*radiation effects , Endothelial Cells/*cytology , Fetal Blood/*cytology, Adult ; Animals ; Cell Cycle/radiation effects ; Cell Separation ; Dose-Response Relationship, Drug ; Humans ; Mice ; Radiation Tolerance/radiation effects ; Time Factors ; X-Rays/adverse effects |
| مستخلص: | Endothelial colony-forming cells (ECFCs) are endothelial progenitor cells that circulate at low concentration in human umbilical cord and adult peripheral blood and are largely resident in blood vessels. ECFCs not only appear to be critical for normal vascular homeostasis and repair but may also contribute to tumor angiogenesis and response to therapy. To begin to characterize the potential role of ECFCs during the treatment of tumors in children and adults with radiation, we characterized the X-ray sensitivity of cord and adult blood-derived ECFCs. We found both cord blood and adult ECFCs to be highly radiation sensitive (3 Gy resulted in >90% killing without induction of apoptosis). The X-ray survival curves suggested reduced potential for repair capacity, but X-ray fractionation studies demonstrated that all the ECFCs exhibited repair when the radiation was fractionated. Finally, the mechanisms of X-ray-induced cell death for cord blood and adult ECFCs were different at low and high dose. At low dose, all ECFCs appear to die by mitotic death/catastrophe. However, at high radiation doses (≥ 10 Gy) cord blood ECFCs underwent p53 stabilization and Bax-dependent apoptosis as well as p21-dependent G₁ and G₂/M cell cycle checkpoints. By contrast, after 10 Gy adult ECFCs undergo only large-scale radiation-induced senescence, which is a cellular phenotype linked to premature development of atherosclerosis and vasculopathies. These data demonstrate that the ECFC response to radiation is dose-dependent and developmentally regulated and may provide potential mechanistic insight into their role in tumor and normal tissue response after ionizing radiation treatment. |
| تواريخ الأحداث: | Date Created: 20110614 Date Completed: 20110922 Latest Revision: 20190918 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1667/rr2427.1 |
| PMID: | 21663393 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1938-5404 |
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| DOI: | 10.1667/rr2427.1 |