دورية أكاديمية
أعرض في EDS

Poly (A)+ transcriptome assessment of ERBB2-induced alterations in breast cell lines.

التفاصيل البيبلوغرافية
العنوان: Poly (A)+ transcriptome assessment of ERBB2-induced alterations in breast cell lines.
المؤلفون: Carraro DM; Centro Internacional de Ensino e Pesquisa, Hospital AC Camargo, São Paulo, São Paulo, Brazil. dirce.carraro@accamargo.org.br, Ferreira EN, de Campos Molina G, Puga RD, Abrantes EF, Trapé AP, Eckhardt BL, Nunes DN, Brentani MM, Arap W, Pasqualini R, Brentani H, Dias-Neto E, Brentani RR
المصدر: PloS one [PLoS One] 2011; Vol. 6 (6), pp. e21022. Date of Electronic Publication: 2011 Jun 22.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Gene Expression Profiling*, Breast/*cytology , Breast/*metabolism , Poly A/*metabolism , Receptor, ErbB-2/*metabolism, Alternative Splicing/genetics ; Base Sequence ; Cell Line ; Computational Biology ; Female ; Gene Fusion/genetics ; Gene Library ; Genome, Human/genetics ; Humans ; Polymorphism, Single Nucleotide/genetics ; Receptor, ErbB-2/genetics ; Reproducibility of Results
مستخلص: We report the first quantitative and qualitative analysis of the poly (A)⁺ transcriptome of two human mammary cell lines, differentially expressing (human epidermal growth factor receptor) an oncogene over-expressed in approximately 25% of human breast tumors. Full-length cDNA populations from the two cell lines were digested enzymatically, individually tagged according to a customized method for library construction, and simultaneously sequenced by the use of the Titanium 454-Roche-platform. Comprehensive bioinformatics analysis followed by experimental validation confirmed novel genes, splicing variants, single nucleotide polymorphisms, and gene fusions indicated by RNA-seq data from both samples. Moreover, comparative analysis showed enrichment in alternative events, especially in the exon usage category, in ERBB2 over-expressing cells, data indicating regulation of alternative splicing mediated by the oncogene. Alterations in expression levels of genes, such as LOX, ATP5L, GALNT3, and MME revealed by large-scale sequencing were confirmed between cell lines as well as in tumor specimens with different ERBB2 backgrounds. This approach was shown to be suitable for structural, quantitative, and qualitative assessment of complex transcriptomes and revealed new events mediated by ERBB2 overexpression, in addition to potential molecular targets for breast cancer that are driven by this oncogene.
التعليقات: Erratum in: PLoS One. 2011;6(7). doi:10.1371/annotation/b8888ae6-e402-43af-a425-6fd5e58deef6. Ekhardt, Bedrich L [corrected to Eckhardt, Bedrich L].
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المشرفين على المادة: 24937-83-5 (Poly A)
EC 2.7.10.1 (ERBB2 protein, human)
EC 2.7.10.1 (Receptor, ErbB-2)
تواريخ الأحداث: Date Created: 20110707 Date Completed: 20111102 Latest Revision: 20211020
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC3120832
DOI: 10.1371/journal.pone.0021022
PMID: 21731642
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0021022