دورية أكاديمية
Solid-phase extraction and quantitative measurement of omega and omega-1 metabolites of JWH-018 and JWH-073 in human urine.
| العنوان: | Solid-phase extraction and quantitative measurement of omega and omega-1 metabolites of JWH-018 and JWH-073 in human urine. |
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| المؤلفون: | Chimalakonda KC; Department of Pharmacology & Toxicology, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, United States., Moran CL, Kennedy PD, Endres GW, Uzieblo A, Dobrowolski PJ, Fifer EK, Lapoint J, Nelson LS, Hoffman RS, James LP, Radominska-Pandya A, Moran JH |
| المصدر: | Analytical chemistry [Anal Chem] 2011 Aug 15; Vol. 83 (16), pp. 6381-8. Date of Electronic Publication: 2011 Jul 27. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Chemical Society. |
| مواضيع طبية MeSH: | Chromatography, Liquid/*methods , Illicit Drugs/*urine , Indoles/*urine , Naphthalenes/*urine , Solid Phase Extraction/*methods , Substance Abuse Detection/*methods , Tandem Mass Spectrometry/*methods, Deuterium/chemistry ; Humans ; Reference Standards ; Sensitivity and Specificity ; Staining and Labeling |
| مستخلص: | The aminoalkylindole agonists JWH-018 and JWH-073 are contained in "K2/SPICE" products sold as "legal marijuana". Previous human metabolic studies have identified (ω)-hydroxyl and (ω)-carboxyl metabolites as biomarkers that are indicative of product use. However, other primary metabolites exhibiting similar chromatographic properties and mass spectra are also excreted in human urine. Analytical standards were used in this study to identify new primary metabolites as (ω-1)-hydroxyl derivatives of JWH-018 and JWH-073. The liquid chromatography tandem mass spectrometry (LC-MS/MS) procedure, coupled with an automated solid-phase extraction procedure incorporating deuterium-labeled internal standards, provides rapid resolution of the (ω)- and (ω-1) metabolites with adequate sensitivity, precision, and accuracy for trace analysis in human urine. Results from four urine specimens collected after individuals reportedly self-administered either JWH-018 or a mixture of JWH-018 and JWH-073 showed the following: (1) all tested metabolites were excreted in high concentrations, (2) (ω)- and (ω-1)-hydroxyl metabolites were exclusively excreted as glucuronic acid conjugates, and (3) ∼5%-80% of the (ω)-carboxyl metabolites was excreted as glucuronic acid conjugates. This is the first report to identify and quantify (ω-1)-hydroxyl metabolites of JWH-018 and JWH-073 and the first to incorporate automated extraction procedures using deuterium-labeled internal standards. Full clinical validation awaits further testing. |
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| معلومات مُعتمدة: | R01 GM075893 United States GM NIGMS NIH HHS; UL1 RR029884-03 United States RR NCRR NIH HHS; U90/CCU616974-07 United States PHS HHS; 1UL1RR029884 United States RR NCRR NIH HHS; R01 GM075893-08 United States GM NIGMS NIH HHS; UL1 RR029884-04 United States RR NCRR NIH HHS; 200-2007-21729 United States PHS HHS; UL1 RR029884 United States RR NCRR NIH HHS; R01-GM075893 United States GM NIGMS NIH HHS |
| المشرفين على المادة: | 0 (Illicit Drugs) 0 (Indoles) 0 (Naphthalenes) AR09D82C7G (Deuterium) G391998J57 (1-pentyl-3-(1-naphthoyl)indole) |
| تواريخ الأحداث: | Date Created: 20110712 Date Completed: 20111206 Latest Revision: 20240414 |
| رمز التحديث: | 20240414 |
| مُعرف محوري في PubMed: | PMC3155992 |
| DOI: | 10.1021/ac201377m |
| PMID: | 21740038 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-6882 |
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| DOI: | 10.1021/ac201377m |