دورية أكاديمية
Vpu-mediated tetherin antagonism of ongoing HIV-1 infection in CD4(+) T-cells is not directly related to the extent of tetherin cell surface downmodulation.
| العنوان: | Vpu-mediated tetherin antagonism of ongoing HIV-1 infection in CD4(+) T-cells is not directly related to the extent of tetherin cell surface downmodulation. |
|---|---|
| المؤلفون: | Kuhl BD; McGill University AIDS Center, Lady Davis Institute, Jewish General Hospital, Montréal, Canada. bjorn.kuhl@mail.mcgill.ca, Sloan RD, Donahue DA, Liang C, Wainberg MA |
| المصدر: | Virology [Virology] 2011 Sep 01; Vol. 417 (2), pp. 353-61. Date of Electronic Publication: 2011 Jul 14. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0110674 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0341 (Electronic) Linking ISSN: 00426822 NLM ISO Abbreviation: Virology Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Host-Pathogen Interactions*, CD4-Positive T-Lymphocytes/*virology , HIV-1/*growth & development , Human Immunodeficiency Virus Proteins/*metabolism , Viral Regulatory and Accessory Proteins/*metabolism, Antigens, CD ; Cells, Cultured ; GPI-Linked Proteins/antagonists & inhibitors ; HIV-1/immunology ; Humans ; Virus Release |
| مستخلص: | Tetherin is a host cell restriction factor that acts against HIV-1 and other enveloped viruses. The antiviral activity of tetherin is antagonized by the HIV-1 protein Vpu, that downregulates tetherin from the cell surface. Here, we report the specific detection of cell surface tetherin levels in primary activated CD4(+) T-cells and in CD4(+) T-cell lines. Differences were observed regarding tetherin cell surface expression, Vpu-mediated tetherin downmodulation and promotion of virus release. However, Vpu expression in all T-cell lines resulted in a 2-fold increase in numbers of infected cells after three days. This implies a Vpu-mediated effect in ongoing infection and possibly in cell-to-cell viral spread that is independent of the extent of Vpu-mediated tetherin cell surface downmodulation. Endogenous cell surface tetherin levels in T-cell lines were also downmodulated following infection with Vpu-deleted virus, suggesting an additional Vpu-independent mechanism of tetherin cell surface downmodulation following HIV-1 infection in T-cell lines. (Copyright © 2011 Elsevier Inc. All rights reserved.) |
| معلومات مُعتمدة: | Canada Canadian Institutes of Health Research |
| المشرفين على المادة: | 0 (Antigens, CD) 0 (BST2 protein, human) 0 (GPI-Linked Proteins) 0 (Human Immunodeficiency Virus Proteins) 0 (Viral Regulatory and Accessory Proteins) 0 (vpu protein, Human immunodeficiency virus 1) |
| تواريخ الأحداث: | Date Created: 20110716 Date Completed: 20111018 Latest Revision: 20110829 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.virol.2011.06.012 |
| PMID: | 21757214 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1096-0341 |
|---|---|
| DOI: | 10.1016/j.virol.2011.06.012 |