دورية أكاديمية
Neuroglobin is up-regulated in the cerebellum of pups exposed to maternal epileptic seizures.
| العنوان: | Neuroglobin is up-regulated in the cerebellum of pups exposed to maternal epileptic seizures. |
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| المؤلفون: | Lima DC; Departamento de Neurologia e Neurocirurgia, Disciplina de Neurologia Experimental/Universidade Federal de São Paulo, UNIFESP, Rua Botucatu, 862, Brazil., Cossa AC, Perosa SR, de Oliveira EM, da Silva JA Jr, da Silva Fernandes MJ, da Silva IR, Higa EM, da Graça Naffah-Mazzacoratti M, Cavalheiro EA, Amado D |
| المصدر: | International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience [Int J Dev Neurosci] 2011 Dec; Vol. 29 (8), pp. 891-7. Date of Electronic Publication: 2011 Jul 13. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: John Wiley & Sons, Inc Country of Publication: United States NLM ID: 8401784 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-474X (Electronic) Linking ISSN: 07365748 NLM ISO Abbreviation: Int J Dev Neurosci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2020- : Hoboken, NJ : John Wiley & Sons, Inc. Original Publication: Oxford : New York : Pergamon Press, c1983- |
| مواضيع طبية MeSH: | Prenatal Exposure Delayed Effects*, Cerebellum/*metabolism , Epilepsy/*physiopathology , Globins/*metabolism , Nerve Tissue Proteins/*metabolism, Animals ; Animals, Newborn ; Epilepsy/chemically induced ; Female ; Glial Fibrillary Acidic Protein/genetics ; Glial Fibrillary Acidic Protein/metabolism ; Globins/genetics ; Hypoxia-Ischemia, Brain/metabolism ; Muscarinic Agonists/pharmacology ; Nerve Tissue Proteins/genetics ; Neuroglobin ; Nitric Oxide/metabolism ; Pilocarpine/pharmacology ; Pregnancy ; Rats ; Rats, Wistar ; Thiobarbituric Acid Reactive Substances/metabolism ; Up-Regulation |
| مستخلص: | To evaluate a potential insult in the cerebellum of pups exposed to maternal epileptic seizures during intrauterine life, female rats were subjected to pilocarpine-induced epilepsy. Pups from different litters were sacrificed at 1, 3, 7 and 14 post-natal days (PN) and neuroglobin (Ngb) and gliosis were analyzed in the cerebellum by Western blotting (WB) and RT-PCR. (14)C-l-leucine-[(14)C-Leu] incorporation was used to analyze protein synthesis at PN1. Nitric Oxide (NO) and thiobarbituric acid-reactive substances (TBARS) levels were also measured. Pups from naive mothers were used as controls. The mRNA level of Ngb was increased in experimental animals at PN1 ((**)p ≤ 0.001) and PN3 ((**)p ≤ 0.001), at PN7 ((***)p ≤ 0.0001) and at PN14 ((**)p ≤ 0.001) compared to the respective controls. The protein level of Ngb increased significantly in the experimental pups at PN1 ((*)p ≤ 0.05) and at PN3 ((**)p ≤ 0.001), when compared to the control pups at PN1 and PN3. At PN7 and PN14 no difference was found. The mRNA level of GFAP increased significantly about two times at PN3 ((*)p ≤ 0.05) and PN7 ((*)p ≤ 0.05) in the experimental pups when compared to the respective controls, but was unchanged in the other studied ages. Data showed that experimental pups at PN1 exhibited reduced (about 2 times, (*)p ≤ 0.05) total protein synthesis in the cerebellum when compared to control. No differences were found in the NO and TBARS levels. Our data support the hypothesis that an up-regulation of Ngb could be a compensatory mechanism in response to the hypoxic-ischemic insults caused by seizures in pups during intrauterine life. (Copyright © 2011 ISDN. Published by Elsevier Ltd. All rights reserved.) |
| المشرفين على المادة: | 0 (Glial Fibrillary Acidic Protein) 0 (Muscarinic Agonists) 0 (Nerve Tissue Proteins) 0 (Neuroglobin) 0 (Thiobarbituric Acid Reactive Substances) 01MI4Q9DI3 (Pilocarpine) 31C4KY9ESH (Nitric Oxide) 9004-22-2 (Globins) |
| تواريخ الأحداث: | Date Created: 20110720 Date Completed: 20120423 Latest Revision: 20200106 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.ijdevneu.2011.07.002 |
| PMID: | 21767627 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-474X |
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| DOI: | 10.1016/j.ijdevneu.2011.07.002 |