دورية أكاديمية
Differential expression of cellular microRNAs in HPV 11, -16, and -45 transfected cells.
| العنوان: | Differential expression of cellular microRNAs in HPV 11, -16, and -45 transfected cells. |
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| المؤلفون: | Dreher A; Institute of Cellular and Molecular Medicine, DNA Tumor Virus Laboratory, University of Copenhagen, Panum Institute, Blegdamsvej 3, 2200 Copenhagen, Denmark., Rossing M, Kaczkowski B, Andersen DK, Larsen TJ, Christophersen MK, Nielsen FC, Norrild B |
| المصدر: | Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2011 Aug 19; Vol. 412 (1), pp. 20-5. Date of Electronic Publication: 2011 Jul 18. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2002- >: San Diego, CA : Elsevier Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Gene Expression Regulation, Viral*, Genome, Viral/*genetics , Human papillomavirus 11/*genetics , Human papillomavirus 16/*genetics , MicroRNAs/*genetics , Tumor Virus Infections/*genetics, 3' Untranslated Regions/genetics ; Cell Line ; Humans ; RNA Splicing ; Transfection ; Tumor Virus Infections/virology |
| مستخلص: | Human papillomaviruses (HPVs) are highly prevalent giving rise to both benign and malignant lesions why they are classified as high- and low-risk viruses. In this study we selected one low-risk (HPV 11) and two high-risk (HPV 16 and -45) types for genomewide miRNA analysis to investigate possible common and distinct features in the expression profiles. For this purpose we developed a cell culture model system in HaCaT cells for expression of the viral genomes under standardized conditions. We identified 25 miRNAs which were differentially regulated in two or three HPV types where 13 miRNAs were in common for all three types. Among the miRNAs identified, miR-125a-5p, miR-129-3p, miR-363, and miR-145 are related to human cancers. Noteworthy, miR-145 is found upregulated in the miRNA profiles of both high-risk HPV types. For selected differentially expressed miRNAs in HPV 16 predicted miRNA target transcript involved in signal transduction, RNA splicing and tumor invasive growth were validated by qRT-PCR. In addition, our results imply that the early 3' untranslated region (3'UTR) of the three HPV genomes were not a target for miRNA regulation. (Copyright © 2011 Elsevier Inc. All rights reserved.) |
| المشرفين على المادة: | 0 (3' Untranslated Regions) 0 (MicroRNAs) |
| تواريخ الأحداث: | Date Created: 20110726 Date Completed: 20111014 Latest Revision: 20110822 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.bbrc.2011.07.011 |
| PMID: | 21782796 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2104 |
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| DOI: | 10.1016/j.bbrc.2011.07.011 |