دورية أكاديمية
Selection of Rattus norvegicus oocytes for in vitro maturation by brilliant cresyl blue staining.
| العنوان: | Selection of Rattus norvegicus oocytes for in vitro maturation by brilliant cresyl blue staining. |
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| المؤلفون: | Alcoba DD; Laboratório de Biologia Celular-Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA)-Porto Alegre, Brazil., da Rosa Braga BL, Sandi-Monroy NL, Proença LA, Felix Lopes RF, de Oliveira AT |
| المصدر: | Zygote (Cambridge, England) [Zygote] 2013 Aug; Vol. 21 (3), pp. 238-45. Date of Electronic Publication: 2011 Jul 27. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cambridge University Press Country of Publication: England NLM ID: 9309124 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-8730 (Electronic) Linking ISSN: 09671994 NLM ISO Abbreviation: Zygote Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge ; New York, NY : Cambridge University Press, c1993- |
| مواضيع طبية MeSH: | In Vitro Oocyte Maturation Techniques*, Oocytes/*physiology , Oxazines/*analysis , Staining and Labeling/*methods, Animals ; Coloring Agents/analysis ; Coloring Agents/pharmacology ; Cumulus Cells ; Dose-Response Relationship, Drug ; Female ; Meiosis ; Oocytes/cytology ; Oocytes/drug effects ; Oxazines/pharmacology ; Rats ; Rats, Wistar ; Time Factors |
| مستخلص: | The objective of this work was to evaluate the rate of meiosis resumption and nuclear maturation of rat (Rattus norvegicus) oocytes selected for in vitro maturation (IVM) after staining of cumulus-oocyte complexes (COCs) with blue cresyl brilliant (BCB) using different protocols: exposure for 30, 60 or 90 min at 26 μM BCB (Experiment 1), and exposure for 60 min at 13, 20 or 26 μM BCB (Experiment 2). In Experiment 1, the selection of oocytes exposed to BCB for 60 min was found to be the most suitable, as meiosis resumption rates in the BCB(+) group (n = 35/61; 57.37%) were the closest to the observed in the control (not exposed) group (n = 70/90; 77.77%) and statistically higher than the values observed for the BCB(-) group (n = 3/41; 7.32%). Additionally, the more effective evaluation of diagnostic tests (sensitivity and negative predictive value 100%) was observed in COCs exposed for 60 min. In Experiment 2, the 13 μM BCB(+) group presented rates of meiosis resumption (n = 57/72; 72.22%) similar to the control group (n = 87/105; 82.86%) and higher than other concentration groups. However, this results of the analysis between BCB(-) oocytes was also higher in the 13 μM BCB group (n = 28/91; 30.78%) when compared with BCB(-) COCs exposed to 20 μM (n = 3/62; 4.84%) or 26 μM (n = 3/61; 4.92%) BCB. The nuclear maturation rate in the 13 μM BCB group was similar between BCB(+) or BCB(-) oocytes. The 20 μM BCB group had a lower rate of nuclear maturation of BCB(-) oocytes than other groups. Thus, our best results in the selection of Rattus norvegicus oocytes by staining with BCB were obtained using the concentration of 13 μM and 20 μM, and an incubation period of 60 min. |
| المشرفين على المادة: | 0 (Coloring Agents) 0 (Oxazines) 4712-70-3 (Brilliant Cresyl Blue) |
| تواريخ الأحداث: | Date Created: 20110728 Date Completed: 20140131 Latest Revision: 20130621 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1017/S0967199411000463 |
| PMID: | 21791168 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1469-8730 |
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| DOI: | 10.1017/S0967199411000463 |