دورية أكاديمية
Further analysis of the T cell receptor gamma/delta+ peripheral lymphocyte subset. The V delta 1 gene segment is expressed with either C alpha or C delta.
| العنوان: | Further analysis of the T cell receptor gamma/delta+ peripheral lymphocyte subset. The V delta 1 gene segment is expressed with either C alpha or C delta. |
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| المؤلفون: | Miossec C; Laboratoire d'Immunologie Cellulaire, Institut Gustave-Roussy, Villejuif, France., Faure F, Ferradini L, Roman-Roman S, Jitsukawa S, Ferrini S, Moretta A, Triebel F, Hercend T |
| المصدر: | The Journal of experimental medicine [J Exp Med] 1990 Apr 01; Vol. 171 (4), pp. 1171-88. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Rockefeller University Press Country of Publication: United States NLM ID: 2985109R Publication Model: Print Cited Medium: Print ISSN: 0022-1007 (Print) Linking ISSN: 00221007 NLM ISO Abbreviation: J Exp Med Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, NY : Rockefeller University Press |
| مواضيع طبية MeSH: | Receptors, Antigen, T-Cell/*genetics , T-Lymphocytes/*immunology, Animals ; Antibodies, Monoclonal ; Blotting, Northern ; Blotting, Southern ; Cell Line ; Cytotoxicity, Immunologic ; DNA/genetics ; DNA/isolation & purification ; DNA Probes ; Flow Cytometry ; Fluorescent Antibody Technique ; Humans ; Macromolecular Substances ; Mice ; Mice, Inbred Strains/immunology ; Phenotype ; Receptors, Antigen, T-Cell/analysis ; T-Lymphocytes/cytology |
| مستخلص: | In the present study, we have characterized the reactivity of two mAbs that are directed at the human TCR-gamma/delta. These reagents, designated anti-A13 and anti-TiV delta 2, were found to recognize antigenic determinants encoded by the TCR V delta 1 and V delta 2 gene segments, respectively. Immunofluorescence analyses performed with the antibodies confirmed that, in the TCR-gamma/delta+ cell subpopulation, the expression of V delta 2+ delta chains is largely predominant, as compared with the V delta 1+ counterparts. However, these experiments led to an apparently discrepant finding. Indeed, the total number of cells recognized by the anti-A13 plus the anti-TiV delta 2 antibodies was often greater than that detected with anti-TCR-delta 1, a reagent specific for a constant epitope of the human delta chain. Further investigation showed that the presence of a sizeable peripheral lymphocyte subset coexpressing the BMA031 and the A13 epitopes. Because the former antibody is known to recognize an invariant antigenic determinant of the TCR-alpha/beta dimer, these results suggested that the V delta 1 gene segment may be expressed with either C delta or C alpha. This hypothesis was confirmed using T2, an IL-2-dependent BMA031+ A13+ polyclonal cell line developed from peripheral blood of a healthy adult donor. Indeed, T2 cells were found to have productively rearranged the V delta 1 gene. Together, results of Northern blot analysis and cDNA cloning indicated that V delta 1 was expressed in these cells as part of a 1.6-kb full-length message including J alpha-C alpha segments. |
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| المشرفين على المادة: | 0 (Antibodies, Monoclonal) 0 (DNA Probes) 0 (Macromolecular Substances) 0 (Receptors, Antigen, T-Cell) 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 19900401 Date Completed: 19900517 Latest Revision: 20190508 |
| رمز التحديث: | 20240829 |
| مُعرف محوري في PubMed: | PMC2187822 |
| DOI: | 10.1084/jem.171.4.1171 |
| PMID: | 2182762 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0022-1007 |
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| DOI: | 10.1084/jem.171.4.1171 |