دورية أكاديمية
Validation of an internally controlled one-step real-time multiplex RT-PCR assay for the detection and quantitation of dengue virus RNA in plasma.
| العنوان: | Validation of an internally controlled one-step real-time multiplex RT-PCR assay for the detection and quantitation of dengue virus RNA in plasma. |
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| المؤلفون: | Hue KD; Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben Ham Tu, Quan 5, Ho Chi Minh City, Viet Nam. kiendth@oucru.org, Tuan TV, Thi HT, Bich CT, Anh HH, Wills BA, Simmons CP |
| المصدر: | Journal of virological methods [J Virol Methods] 2011 Nov; Vol. 177 (2), pp. 168-73. Date of Electronic Publication: 2011 Aug 05. |
| نوع المنشور: | Journal Article; Validation Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Amsterdam] : Elsevier/North-Holland Biomedical Press, 1980- |
| مواضيع طبية MeSH: | Dengue/*diagnosis , Dengue Virus/*genetics , Multiplex Polymerase Chain Reaction/*methods , RNA, Viral/*blood , Reverse Transcriptase Polymerase Chain Reaction/*methods, Dengue/virology ; Equartevirus/genetics ; Humans ; Limit of Detection ; Microbiological Techniques ; Plasmids/genetics ; Plasmids/metabolism ; Reproducibility of Results ; Sensitivity and Specificity ; Viral Nonstructural Proteins/genetics |
| مستخلص: | Dengue is mosquito-borne virus infection that annually causes ~50 million clinically apparent cases worldwide. An internally controlled one-step real-time multiplex RT-PCR assay was developed for detection and quantitation of DENV RNA in plasma sample by using specific primers and fluorogenic TaqMan probes. All primers and probes targeted sequences near the 3' end of the NS5 gene. The method comprised two multiplex assays and was validated for sensitivity, specificity, linearity, reproducibility and precision. An internal control template was spiked into each clinical specimen to provide quality assurance for each experimental step. The assay allowed for detection of between 0.5 and 3 infectious particles per mL, is rapid and has been operationally characterized in 287 Vietnamese dengue patients from two therapeutic intervention trials at the Hospital for Tropical Diseases, Ho Chi Minh City, Viet Nam. (Copyright © 2011 Elsevier B.V. All rights reserved.) |
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| معلومات مُعتمدة: | 084368 United Kingdom WT_ Wellcome Trust |
| فهرسة مساهمة: | Indexing Agency: NLM Local ID #: EMS62282. |
| المشرفين على المادة: | 0 (RNA, Viral) 0 (Viral Nonstructural Proteins) |
| تواريخ الأحداث: | Date Created: 20110817 Date Completed: 20120119 Latest Revision: 20240512 |
| رمز التحديث: | 20240512 |
| مُعرف محوري في PubMed: | PMC4347661 |
| DOI: | 10.1016/j.jviromet.2011.08.002 |
| PMID: | 21843553 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0984 |
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| DOI: | 10.1016/j.jviromet.2011.08.002 |