دورية أكاديمية
Molecular assay optimized by Taguchi experimental design method for venous thromboembolism investigation.
| العنوان: | Molecular assay optimized by Taguchi experimental design method for venous thromboembolism investigation. |
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| المؤلفون: | Celani de Souza HJ; Sao Paulo State University, UNESP, Department of Production Engineering, Guaratinguetá, SP, Brazil. hcelani@uol.com.br, Moyses CB, Pontes FJ, Duarte RN, Sanches da Silva CE, Alberto FL, Ferreira UR, Silva MB |
| المصدر: | Molecular and cellular probes [Mol Cell Probes] 2011 Oct-Dec; Vol. 25 (5-6), pp. 231-7. Date of Electronic Publication: 2011 Aug 17. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: England NLM ID: 8709751 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-1194 (Electronic) Linking ISSN: 08908508 NLM ISO Abbreviation: Mol Cell Probes Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London ; New York : Academic Press, c1987- |
| مواضيع طبية MeSH: | Venous Thromboembolism*/diagnosis , Venous Thromboembolism*/genetics, DNA Mutational Analysis/*methods , Factor V/*genetics , Fluorescence Resonance Energy Transfer/*methods , Prothrombin/*genetics , Real-Time Polymerase Chain Reaction/*methods, Algorithms ; Factor Analysis, Statistical ; Genotype ; Humans ; Mutation |
| مستخلص: | Two mutations - Factor V Leiden (1691G > A) and the 20210G > A on the Prothrombin gene - are key risk factors for a frequent and potentially fatal disorder called Venous Thromboembolism. These molecular alterations can be investigated using real-time Polymerase Chain Reaction (PCR) with Fluorescence Resonance Energy Transfer (FRET) probes and distinct DNA pools for both factors. The objective of this paper is to present an application of Taguchi Experimental Design Method to determine the best parameters adjustment of a Molecular Assays Process in order to obtain the best diagnostic result for Venous Thromboembolism investigation. The complete process contains six three-level factors which usually demands 729 experiments to obtain the final result, if using a Full Factorial Array. In this research, a Taguchi L27 Orthogonal Array is chosen to optimize the analysis and reduce the number of experiments to 27 without degrading the final result accuracy. The application of this method can lessen the time and cost necessary to achieve the best operation condition for a required performance. The results is proven in practice and confirmed that the Taguchi method can really offer a good approach for clinical assay efficiency and effectiveness improvement even though the clinical diagnostics can be based on the use of qualitative techniques. (Copyright © 2011 Elsevier Ltd. All rights reserved.) |
| المشرفين على المادة: | 9001-24-5 (Factor V) 9001-26-7 (Prothrombin) |
| تواريخ الأحداث: | Date Created: 20110827 Date Completed: 20120222 Latest Revision: 20111104 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.mcp.2011.08.001 |
| PMID: | 21867748 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-1194 |
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| DOI: | 10.1016/j.mcp.2011.08.001 |