دورية أكاديمية
Genetically encoded ratiometric biosensors to measure intracellular exchangeable zinc in Escherichia coli.
| العنوان: | Genetically encoded ratiometric biosensors to measure intracellular exchangeable zinc in Escherichia coli. |
|---|---|
| المؤلفون: | Wang D; University of Michigan, Department of Chemistry, Ann Arbor, Michigan 48109, USA. wangda@umich.edu, Hurst TK, Thompson RB, Fierke CA |
| المصدر: | Journal of biomedical optics [J Biomed Opt] 2011 Aug; Vol. 16 (8), pp. 087011. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society Country of Publication: United States NLM ID: 9605853 Publication Model: Print Cited Medium: Internet ISSN: 1560-2281 (Electronic) Linking ISSN: 10833668 NLM ISO Abbreviation: J Biomed Opt Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bellingham, WA : Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society, c1996- |
| مواضيع طبية MeSH: | Biosensing Techniques/*methods , Escherichia coli/*metabolism , Fluorescence Resonance Energy Transfer/*methods , Zinc/*analysis, Biosensing Techniques/instrumentation ; Carbonic Anhydrases/genetics ; Carbonic Anhydrases/metabolism ; Escherichia coli/chemistry ; Escherichia coli/genetics ; Luminescent Proteins/chemistry ; Luminescent Proteins/genetics ; Luminescent Proteins/metabolism ; Models, Biological ; Molecular Biology/methods ; Zinc/metabolism ; Red Fluorescent Protein |
| مستخلص: | Zinc is an essential element for numerous cellular processes, therefore zinc homeostasis is regulated in living organisms. Fluorescent sensors have been developed as important tools to monitor the concentrations of readily exchangeable zinc in live cells. One type of biosensor uses carbonic anhydrase (CA) as the recognition element based on its tunable affinity, superior metal selectivity, and fluorescence signal from aryl sulfonamide ligands coupled to zinc binding. Here, we fuse carbonic anhydrase with a red fluorescent protein to create a series of genetically-encoded Förster resonance energy transfer-based excitation ratiometric zinc sensors that exhibit large signal increases in response to alterations in physiological-free zinc concentrations. These sensors were applied to the prokaryotic model organism Escherichia coli to quantify the readily exchangeable zinc concentration. In minimal media, E. coli BL21(DE3) cells expressing the CA sensor, exhibit a median intracellular readily exchangeable zinc concentration of 20 pM, much less than the total cellular zinc concentration of ∼0.2 mM. Furthermore, the intracellular readily exchangeable zinc concentration varies with the concentration of environmental zinc. |
| References: | Proc Natl Acad Sci U S A. 2000 Jan 4;97(1):477-82. (PMID: 10618443) Biochemistry. 1997 Dec 16;36(50):15780-91. (PMID: 9398308) J Biol Chem. 1997 Aug 15;272(33):20364-72. (PMID: 9252341) J Membr Biol. 1991 Jul;123(1):63-71. (PMID: 1774775) Appl Environ Microbiol. 2006 Jul;72(7):4695-703. (PMID: 16820461) Biochemistry. 1999 Jul 13;38(28):9054-62. (PMID: 10413479) Nat Biotechnol. 2004 Dec;22(12):1567-72. (PMID: 15558047) Biometals. 2001 Sep-Dec;14(3-4):271-313. (PMID: 11831461) J Biol Chem. 2009 Jun 12;284(24):16289-16297. (PMID: 19363034) Biometals. 2006 Oct;19(5):461-71. (PMID: 16937252) Biometals. 2001 Sep-Dec;14(3-4):205-22. (PMID: 11831457) Methods Enzymol. 2008;450:287-309. (PMID: 19152866) Nat Methods. 2009 Oct;6(10):737-40. (PMID: 19718032) Biochim Biophys Acta. 2006 Jul;1763(7):711-22. (PMID: 16675045) J Gen Microbiol. 1979 Nov;115(1):69-77. (PMID: 393798) Nat Methods. 2007 Jul;4(7):555-7. (PMID: 17572680) J Biol Chem. 2010 Oct 29;285(44):33788-96. (PMID: 20709752) J Inorg Biochem. 2004 Apr;98(4):639-48. (PMID: 15041244) J Nutr. 2003 May;133(5 Suppl 1):1532S-5S. (PMID: 12730459) J Am Chem Soc. 2003 Sep 3;125(35):10591-7. (PMID: 12940742) ACS Chem Biol. 2006 Mar 17;1(2):103-11. (PMID: 17163650) J Biomed Opt. 2000 Jan;5(1):17-22. (PMID: 10938761) Biochim Biophys Acta. 2010 Feb;1804(2):393-403. (PMID: 19818877) IUBMB Life. 2002 Dec;54(6):351-5. (PMID: 12665246) Science. 2001 Jun 29;292(5526):2488-92. (PMID: 11397910) J Biol Chem. 1991 Sep 15;266(26):17320-5. (PMID: 1910042) J Physiol. 1933 Dec 5;80(2):113-42. (PMID: 16994489) J Am Chem Soc. 2001 Sep 5;123(35):8614-5. (PMID: 11525677) Mol Pharmacol. 1992 Feb;41(2):419-26. (PMID: 1538718) J Neurosci Methods. 2002 Jul 30;118(1):63-75. (PMID: 12191759) J Biol Chem. 1962 Apr;237:1129-37. (PMID: 14465547) Anal Biochem. 1977 May 1;79(1-2):614-7. (PMID: 405888) J Neurochem. 2003 May;85(3):563-70. (PMID: 12694382) J Bacteriol. 2005 Feb;187(3):1124-34. (PMID: 15659689) J Biol Chem. 1993 Dec 25;268(36):27458-66. (PMID: 8262987) Proc Natl Acad Sci U S A. 1981 Oct;78(10):6271-5. (PMID: 7031646) J Biomed Opt. 2002 Oct;7(4):555-60. (PMID: 12421121) J Biol Chem. 2003 Apr 25;278(17):15065-72. (PMID: 12556516) Physiol Rev. 1993 Jan;73(1):79-118. (PMID: 8419966) Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7877-82. (PMID: 12060735) Biochem Biophys Res Commun. 2002 Aug 30;296(4):923-8. (PMID: 12200136) Curr Opin Struct Biol. 2001 Feb;11(1):39-46. (PMID: 11179890) J Biol Inorg Chem. 2006 Nov;11(8):1049-62. (PMID: 16924557) Biometals. 2001 Sep-Dec;14(3-4):239-49. (PMID: 11831459) Acc Chem Res. 2009 Jan 20;42(1):193-203. (PMID: 18989940) J Bacteriol. 1974 Sep;119(3):736-47. (PMID: 4604283) J Nutr. 2003 May;133(5 Suppl 1):1521S-6S. (PMID: 12730457) |
| معلومات مُعتمدة: | R01 EB003924 United States EB NIBIB NIH HHS |
| المشرفين على المادة: | 0 (Luminescent Proteins) EC 4.2.1.1 (Carbonic Anhydrases) J41CSQ7QDS (Zinc) |
| تواريخ الأحداث: | Date Created: 20110908 Date Completed: 20120119 Latest Revision: 20231213 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC3166341 |
| DOI: | 10.1117/1.3613926 |
| PMID: | 21895338 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1560-2281 |
|---|---|
| DOI: | 10.1117/1.3613926 |