دورية أكاديمية
An ana2/ctp/mud complex regulates spindle orientation in Drosophila neuroblasts.
| العنوان: | An ana2/ctp/mud complex regulates spindle orientation in Drosophila neuroblasts. |
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| المؤلفون: | Wang C; Neuroscience & Behavioral Disorder Program, Duke-National University of Singapore Graduate Medical School Singapore, Singapore, Singapore., Li S, Januschke J, Rossi F, Izumi Y, Garcia-Alvarez G, Gwee SS, Soon SB, Sidhu HK, Yu F, Matsuzaki F, Gonzalez C, Wang H |
| المصدر: | Developmental cell [Dev Cell] 2011 Sep 13; Vol. 21 (3), pp. 520-33. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101120028 Publication Model: Print Cited Medium: Internet ISSN: 1878-1551 (Electronic) Linking ISSN: 15345807 NLM ISO Abbreviation: Dev Cell Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge, Mass. : Cell Press, c2001- |
| مواضيع طبية MeSH: | Cell Cycle Proteins/*metabolism , Drosophila Proteins/*metabolism , Drosophila melanogaster/*metabolism , Dyneins/*metabolism , Membrane Proteins/*metabolism , Nerve Tissue Proteins/*metabolism , Neural Stem Cells/*metabolism , Spindle Apparatus/*metabolism, Animals ; Brain/growth & development ; Brain/metabolism ; Cell Division ; Centrioles/metabolism ; Centrosome/metabolism ; Drosophila Proteins/genetics ; Dyneins/genetics ; Guanine Nucleotide Dissociation Inhibitors/metabolism ; Larva/metabolism ; Nerve Tissue Proteins/genetics ; Protein Binding/genetics |
| مستخلص: | Drosophila neural stem cells, larval brain neuroblasts (NBs), align their mitotic spindles along the apical/basal axis during asymmetric cell division (ACD) to maintain the balance of self-renewal and differentiation. Here, we identified a protein complex composed of the tumor suppressor anastral spindle 2 (Ana2), a dynein light-chain protein Cut up (Ctp), and Mushroom body defect (Mud), which regulates mitotic spindle orientation. We isolated two ana2 alleles that displayed spindle misorientation and NB overgrowth phenotypes in larval brains. The centriolar protein Ana2 anchors Ctp to centrioles during ACD. The centriolar localization of Ctp is important for spindle orientation. Ana2 and Ctp localize Mud to the centrosomes and cell cortex and facilitate/maintain the association of Mud with Pins at the apical cortex. Our findings reveal that the centrosomal proteins Ana2 and Ctp regulate Mud function to orient the mitotic spindle during NB asymmetric division. (Copyright © 2011 Elsevier Inc. All rights reserved.) |
| المشرفين على المادة: | 0 (Ana2 protein, Drosophila) 0 (Cell Cycle Proteins) 0 (Drosophila Proteins) 0 (Guanine Nucleotide Dissociation Inhibitors) 0 (Membrane Proteins) 0 (Mud protein, Drosophila) 0 (Nerve Tissue Proteins) 0 (Pins protein, Drosophila) 0 (ctp protein, Drosophila) EC 3.6.4.2 (Dyneins) |
| تواريخ الأحداث: | Date Created: 20110917 Date Completed: 20111123 Latest Revision: 20190606 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.devcel.2011.08.002 |
| PMID: | 21920316 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1878-1551 |
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| DOI: | 10.1016/j.devcel.2011.08.002 |