دورية أكاديمية
[Verification of a decrease in the rigidity of the phage lambda DNA polymeric chain in low ionic strength aqueous solutions by testing the polymer-polymer interlink interactions].
| العنوان: | [Verification of a decrease in the rigidity of the phage lambda DNA polymeric chain in low ionic strength aqueous solutions by testing the polymer-polymer interlink interactions]. |
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| المؤلفون: | Arutiunian AV, Ivanova MA, Kurliand DI, Kapshin IuS, Landa SB, Poshekhonov ST, Drobchenko EA, Shevelev IV |
| المصدر: | Biofizika [Biofizika] 2011 Jul-Aug; Vol. 56 (4), pp. 653-60. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Izdatelstvo Nauka Country of Publication: Russia (Federation) NLM ID: 0372666 Publication Model: Print Cited Medium: Print ISSN: 0006-3029 (Print) Linking ISSN: 00063029 NLM ISO Abbreviation: Biofizika Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Moskva : Izdatelstvo Nauka |
| مواضيع طبية MeSH: | Nucleic Acid Conformation*, Bacteriophage lambda/*chemistry , DNA, Circular/*chemistry , DNA, Viral/*chemistry, Osmolar Concentration |
| مستخلص: | Changes in the rigidity of the polymetric chain of phage lambda double-strand DNA have been studied by laser correlation spectroscopy. It was shown that, as the ionic strength increases, the effect of the screening of the hydrodynamic interaction of the links of the polymeric chain specific for polymeric coils arises in a DNA solution. It is assumed that the screening occurs when the threshold of the overlapping of DNA coils is achieved. The overlapping of coils is the result of a previously observed significant rise of DNA coil size from abnormally small DNA coils in low ionic strength buffers (about 10(-2) M Na+ or less) to maximum possible large coils in the 5SSC and 5SSC-like buffers. Further analysis of the far interlink interactions in linear lambda phage DNA coils in similar buffers at pH 7 and 4 confirms the earlier proposal about the role of H+ ions in the appearance of abnormally small DNA coils. The abnormal decrease in the DNA coil size in low ionic strength buffers is not a specific feature of lambda phage DNA only. |
| المشرفين على المادة: | 0 (DNA, Circular) 0 (DNA, Viral) |
| تواريخ الأحداث: | Date Created: 20110929 Date Completed: 20111018 Latest Revision: 20110928 |
| رمز التحديث: | 20240628 |
| PMID: | 21950067 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0006-3029 |
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