دورية أكاديمية
Decreased insulin sensitivity and increased oxidative damage in wasting adipose tissue depots of wild-type mice.
العنوان: | Decreased insulin sensitivity and increased oxidative damage in wasting adipose tissue depots of wild-type mice. |
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المؤلفون: | Sackmann-Sala L; Edison Biotechnology Institute, Ohio University, 1 Water Tower Dr., The Ridges, Athens, OH 45701, USA., Berryman DE, Lubbers ER, Vesel CB, Troike KM, List EO, Munn RD, Ikeno Y, Kopchick JJ |
المصدر: | Age (Dordrecht, Netherlands) [Age (Dordr)] 2012 Oct; Vol. 34 (5), pp. 1225-37. Date of Electronic Publication: 2011 Sep 29. |
نوع المنشور: | Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
اللغة: | English |
بيانات الدورية: | Publisher: Springer Country of Publication: Netherlands NLM ID: 101250497 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1574-4647 (Electronic) Linking ISSN: 01619152 NLM ISO Abbreviation: Age (Dordr) Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Dordrecht, The Netherlands ; Hingham, MA : Springer, c2005- |
مواضيع طبية MeSH: | Oxidative Stress*, Adipose Tissue/*metabolism , Adipose Tissue, White/*metabolism , Aging/*physiology , Insulin/*metabolism , Insulin Resistance/*physiology , Obesity/*metabolism, Adipocytes/metabolism ; Adipose Tissue, White/pathology ; Animals ; Disease Models, Animal ; Male ; Mice ; Mice, Inbred C57BL ; Obesity/pathology ; Proteomics |
مستخلص: | Unintentional weight loss (wasting) in the elderly is a major health concern as it leads to increased mortality. Several studies have focused on muscle loss, but little is known about the mechanisms giving rise to loss of fat mass at old ages. To investigate potential mechanisms, white adipose tissue (WAT) characteristics and proteomic profiles were compared between adult (10-12-month-old) and aged (22-24-month-old) wild-type mice. Four individual WAT depots were analyzed to account for possible depot-specific differences. Proteomic profiles of WAT depots, along with body weights and compositions, plasma levels of insulin, leptin and adiponectin, insulin tolerance, adipocyte sizes, and products of oxidative damage in each WAT depot were determined. We found that lean mass remained constant while fat mass and insulin tolerance were decreased in old age, as were adipocyte sizes in the WAT depots. Proteomic results showed increased levels of enolase, pyruvate dehydrogenase E1β, NAD(+)-dependent isocitrate dehydrogenase α, and ATP synthase subunit β, and decreased levels of carbonic anhydrase 3 in WAT of aged mice. These data suggest increased aerobic glucose oxidation in wasting WAT, consistent with decreased insulin signaling. Also, Cu/Zn superoxide dismutase and two chaperones were increased in aged WAT depots, indicating higher stress resistance. In agreement, lipid peroxidation (HNE-His adducts) increased in old age, although protein oxidation (carbonyl groups) showed no increase. In conclusion, features of wasting WAT were similar in the four depots, including decreased adipocyte sizes and alterations in protein expression profiles that indicated decreased insulin sensitivity and increased lipid peroxidation. |
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معلومات مُعتمدة: | DK075436-01 United States DK NIDDK NIH HHS; R01 AG019899 United States AG NIA NIH HHS; AG019899-06 United States AG NIA NIH HHS; P01 AG031736 United States AG NIA NIH HHS; R15 DK075436 United States DK NIDDK NIH HHS; 1P01AG031736-01A1 United States AG NIA NIH HHS |
المشرفين على المادة: | 0 (Insulin) |
تواريخ الأحداث: | Date Created: 20110929 Date Completed: 20130312 Latest Revision: 20211020 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC3448990 |
DOI: | 10.1007/s11357-011-9304-7 |
PMID: | 21953241 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1574-4647 |
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DOI: | 10.1007/s11357-011-9304-7 |