دورية أكاديمية
Immunization with Ehrlichia P28 outer membrane proteins confers protection in a mouse model of ehrlichiosis.
| العنوان: | Immunization with Ehrlichia P28 outer membrane proteins confers protection in a mouse model of ehrlichiosis. |
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| المؤلفون: | Crocquet-Valdes PA; Department of Pathology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555-0609, USA., Thirumalapura NR, Ismail N, Yu X, Saito TB, Stevenson HL, Pietzsch CA, Thomas S, Walker DH |
| المصدر: | Clinical and vaccine immunology : CVI [Clin Vaccine Immunol] 2011 Dec; Vol. 18 (12), pp. 2018-25. Date of Electronic Publication: 2011 Oct 26. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101252125 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1556-679X (Electronic) Linking ISSN: 1556679X NLM ISO Abbreviation: Clin Vaccine Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, D.C. : American Society for Microbiology, [2006]- |
| مواضيع طبية MeSH: | Bacterial Outer Membrane Proteins/*immunology , Bacterial Vaccines/*immunology , Ehrlichia/*immunology , Ehrlichiosis/*prevention & control , Immunization/*methods , Vaccines, DNA/*immunology, Animals ; Bacterial Load ; Bacterial Outer Membrane Proteins/genetics ; Bacterial Vaccines/administration & dosage ; Bacterial Vaccines/genetics ; CD4-Positive T-Lymphocytes/immunology ; Ehrlichia/genetics ; Ehrlichiosis/immunology ; Humans ; Liver/microbiology ; Mice ; Spleen/microbiology ; Th1 Cells/immunology ; United States ; Vaccines, DNA/administration & dosage ; Vaccines, DNA/genetics ; Vaccines, Subunit/administration & dosage ; Vaccines, Subunit/genetics ; Vaccines, Subunit/immunology |
| مستخلص: | The obligately intracellular bacterium Ehrlichia chaffeensis that resides in mononuclear phagocytes is the etiologic agent of human monocytotropic ehrlichiosis (HME). HME is an emerging and often life-threatening, tick-transmitted infectious disease in the United States. Effective primary immune responses against Ehrlichia infection involve generation of Ehrlichia-specific gamma interferon (IFN-γ)-producing CD4(+) T cells and cytotoxic CD8(+) T cells, activation of macrophages by IFN-γ, and production of Ehrlichia-specific antibodies of the Th1 isotype. Currently, there are no vaccines available against HME. We evaluated the ability of 28-kDa outer membrane proteins (P28-OMP-1) of the closely related Ehrlichia muris to stimulate long-term protective memory T and B cell responses and confer protection in mice. The spleens of mice vaccinated with E. muris P28-9, P28-12, P28-19, or a mixture of these three P28 proteins (P28s) using a DNA prime-protein boost regimen and challenged with E. muris had significantly lower bacterial loads than the spleens of mock-vaccinated mice. Mice immunized with P28-9, P28-12, P28-19, or the mixture induced Ehrlichia-specific CD4(+) Th1 cells. Interestingly, mice immunized with P28-14, orthologs of which in E. chaffeensis and E. canis are primarily expressed in tick cells, failed to lower the ehrlichial burden in the spleen. Immunization with the recombinant P28-19 protein alone also significantly decreased the bacterial load in the spleen and liver compared to those of the controls. Our study reports, for the first time, the protective roles of the Ehrlichia P28-9 and P28-12 proteins in addition to confirming previous reports of the protective ability of P28-19. Partial protection induced by immunization with P28-9, P28-12, and P28-19 against Ehrlichia was associated with the generation of Ehrlichia-specific cell-mediated and humoral immune responses. |
| References: | Infect Immun. 1996 Jun;64(6):2234-9. (PMID: 8675332) Cell Microbiol. 2006 Sep;8(9):1475-87. (PMID: 16922866) Vaccine. 2002 Jan 15;20(7-8):1215-25. (PMID: 11803084) Infect Immun. 2003 May;71(5):2516-24. (PMID: 12704123) Biochem Biophys Res Commun. 1998 Jun 29;247(3):636-43. (PMID: 9647746) Infect Immun. 2007 Jan;75(1):135-45. (PMID: 17060466) J Immunol. 2002 Aug 1;169(3):1419-25. (PMID: 12133967) Infect Immun. 2003 Sep;71(9):5324-31. (PMID: 12933880) Infect Immun. 2005 Jan;73(1):79-87. (PMID: 15618143) Infect Immun. 2008 May;76(5):1920-30. (PMID: 18285501) Ann N Y Acad Sci. 2005 Dec;1063:420-4. (PMID: 16481552) Infect Immun. 2001 Apr;69(4):2083-91. (PMID: 11254561) Parasite Immunol. 2009 Jun;31(6):296-303. (PMID: 19493209) Am J Pathol. 2004 Sep;165(3):997-1006. (PMID: 15331423) Infect Immun. 2002 Apr;70(4):1824-31. (PMID: 11895944) Clin Microbiol Rev. 2003 Jan;16(1):37-64. (PMID: 12525424) Gene. 2004 Apr 14;330:159-68. (PMID: 15087135) Infect Immun. 2003 Jan;71(1):187-95. (PMID: 12496165) Infect Immun. 2007 Oct;75(10):4933-41. (PMID: 17664264) Infect Immun. 2002 Aug;70(8):4701-4. (PMID: 12117987) Infect Immun. 2006 Aug;74(8):4856-64. (PMID: 16861674) Am J Trop Med Hyg. 2001 Oct;65(4):371-8. (PMID: 11693887) Am J Pathol. 2001 Feb;158(2):757-69. (PMID: 11159213) Parasite Immunol. 1998 Mar;20(3):111-9. (PMID: 9568614) Infect Immun. 1998 Jan;66(1):132-9. (PMID: 9423849) J Exp Med. 1996 Jan 1;183(1):271-5. (PMID: 8551231) J Immunol. 2004 Feb 1;172(3):1786-800. (PMID: 14734762) Clin Infect Dis. 2001 Nov 1;33(9):1586-94. (PMID: 11568857) Infect Immun. 1998 Aug;66(8):3892-9. (PMID: 9673277) J Immunol. 2001 Feb 1;166(3):1855-62. (PMID: 11160232) J Immunol. 2004 Jun 1;172(11):6894-901. (PMID: 15153508) Infect Immun. 2007 Nov;75(11):5453-9. (PMID: 17698576) Ann Intern Med. 1994 May 1;120(9):736-43. (PMID: 8147546) Infect Immun. 2004 Feb;72(2):966-71. (PMID: 14742542) Infect Immun. 2004 Aug;72(8):4336-43. (PMID: 15271888) Emerg Infect Dis. 2003 Dec;9(12):1579-86. (PMID: 14720399) |
| معلومات مُعتمدة: | R01 AI031431 United States AI NIAID NIH HHS; R21 AI031431 United States AI NIAID NIH HHS; AI31431 United States AI NIAID NIH HHS |
| المشرفين على المادة: | 0 (Bacterial Outer Membrane Proteins) 0 (Bacterial Vaccines) 0 (Vaccines, DNA) 0 (Vaccines, Subunit) |
| تواريخ الأحداث: | Date Created: 20111028 Date Completed: 20120314 Latest Revision: 20211020 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC3232687 |
| DOI: | 10.1128/CVI.05292-11 |
| PMID: | 22030371 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1556-679X |
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| DOI: | 10.1128/CVI.05292-11 |