دورية أكاديمية
أعرض في EDS

Factors influencing the abundance of the side population in a human myeloma cell line.

التفاصيل البيبلوغرافية
العنوان: Factors influencing the abundance of the side population in a human myeloma cell line.
المؤلفون: Mo SL; The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China., Li J, Loh YS, Brown RD, Smith AL, Chen Y, Joshua D, Roufogalis BD, Li GQ, Fan K, Ng MC, Sze DM
المصدر: Bone marrow research [Bone Marrow Res] 2011; Vol. 2011, pp. 524845. Date of Electronic Publication: 2011 Sep 22.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Hindawi Pub. Corp Country of Publication: Egypt NLM ID: 101566202 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2090-3006 (Electronic) Linking ISSN: 20903006 NLM ISO Abbreviation: Bone Marrow Res Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Cairo : Hindawi Pub. Corp.
مستخلص: Side population (SP) refers to a group of cells, which is capable to efflux Hoechst 33342, a DNA-binding dye. SP cells exist both in normal and tumor tissues. Although SP abundance has been used as an indicator for disease prognostic and drug screening in many research projects, few studies have systematically examined the factors influencing SP analysis. In this study we aim to develop a more thorough understanding of the multiple factors involved in SP analysis including Hoechst 33342 staining and cell culture. RPMI-8226, a high SP percentage (SP%) human myeloma cell line was employed here. The results showed that SP% was subject to staining conditions including: viable cell proportion, dye concentration, staining cell density, incubation duration, staining volume, and mix interval. In addition, SP% was highest in day one after passage, while dropped steadily over time. This study shows that both staining conditions and culture duration can significantly affect SP%. In this case, any conclusions based on SP% should be interpreted cautiously. The relation between culture duration and SP% suggests that the incidence of SP cells may be related to cell proliferation and cell cycle phase. Maintaining these technical variables consistently is essential in SP research.
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تواريخ الأحداث: Date Created: 20111103 Date Completed: 20111110 Latest Revision: 20211021
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC3196862
DOI: 10.1155/2011/524845
PMID: 22046565
قاعدة البيانات: MEDLINE
الوصف
تدمد:2090-3006
DOI:10.1155/2011/524845