دورية أكاديمية
أعرض في EDS

Development of multiplex PCR method for the analysis of glutathione s-transferase polymorphism.

التفاصيل البيبلوغرافية
العنوان: Development of multiplex PCR method for the analysis of glutathione s-transferase polymorphism.
المؤلفون: Kim MS; Department of Pediatrics, Cancer Research Institute, Seoul National University College of Medicine, South Korea., Kang HJ, Park HJ, Yook YJ, Han BD, Kim CW, Kim NH, Lee JW, Kim H, Park KD, Shin HY, Ahn HS
المصدر: Molecular diagnosis & therapy [Mol Diagn Ther] 2011 Oct 01; Vol. 15 (5), pp. 285-92.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Adis, Springer International Country of Publication: New Zealand NLM ID: 101264260 Publication Model: Print Cited Medium: Internet ISSN: 1179-2000 (Electronic) Linking ISSN: 11771062 NLM ISO Abbreviation: Mol Diagn Ther Subsets: MEDLINE
أسماء مطبوعة: Publication: Auckland : Adis, Springer International
Original Publication: Auckland, N.Z. : Adis International, c2006-
مواضيع طبية MeSH: Glutathione Transferase/*genetics , Multiplex Polymerase Chain Reaction/*methods , Polymorphism, Genetic/*genetics, Genotype ; Humans
مستخلص: Background: Busulfan is a key compound in myeloablative chemotherapy before hematopoietic stem-cell transplantation in children. Genetic polymorphisms of glutathione S-transferase (GST), which is involved in the metabolism of busulfan, have been implicated in interindividual variability in busulfan pharmacokinetics. Development of a rapid and simplified method for polygenic analysis of GST may facilitate large pharmacogenetic studies and clinical application of individualized busulfan dose adjustment. We previously introduced an effective PCR method for analyzing multiple genes using a small amount of DNA, termed 'TotalPlex amplification'.
Objective: The aim of this study was to extend the application of the TotalPlex method to the specific GST gene families (A1, P1, M1, and T1) that are related to busulfan metabolism, and thereby facilitate pharmacogenetic analysis of GST polymorphisms.
Methods: Seven genetic polymorphisms (GSTA1 promoter -52G>A, -69C>T, -567T>G, and -631T>G; GSTP1 313A>G; GSTM1 deletion; and GSTT1 deletion) were analyzed by multiplex PCR and genotyping, and the genotyping results from TotalPlex were verified with those from uniplex PCR.
Results: Using five pairs of specific bulging-specific primers, seven specific gene fragments were successfully amplified by multiplex amplification coupled to a multiplexed bead array detection system, with a smaller amount of DNA and a shorter process time than is needed for the conventional approach. The genotypes of seven loci from 30 different genomic DNA samples derived using the multiplex system were consistent with the results of standard genotyping methods.
Conclusion: Our multiplex system provides a fast, inexpensive, and accurate method of detecting multiple GST polymorphisms (GSTA1, GSTP1, GSTM1, and GSTT1).
References: J Biol Chem. 1998 Feb 6;273(6):3517-27. (PMID: 9452477)
Annu Rev Pharmacol Toxicol. 2005;45:51-88. (PMID: 15822171)
Nucleic Acids Res. 2007;35(6):e40. (PMID: 17287288)
Pediatr Nephrol. 2009 Sep;24(9):1773-4. (PMID: 19169714)
Bone Marrow Transplant. 1995 Jul;16(1):31-42. (PMID: 7581127)
J Clin Pharmacol. 2008 Sep;48(9):1052-62. (PMID: 18635758)
Blood. 2004 Sep 1;104(5):1574-7. (PMID: 15142875)
Br J Clin Pharmacol. 2008 Jul;66(1):50-9. (PMID: 18341668)
Blood. 1997 Mar 1;89(5):1701-7. (PMID: 9057653)
Ther Drug Monit. 2008 Aug;30(4):504-10. (PMID: 18641537)
Mol Cell Probes. 2008 Jun;22(3):193-200. (PMID: 18385010)
Clin Pharmacol Ther. 2002 Jun;71(6):479-87. (PMID: 12087351)
Pediatr Blood Cancer. 2010 Dec 1;55(6):1172-9. (PMID: 20672371)
J Biol Chem. 1997 Apr 11;272(15):10004-12. (PMID: 9092542)
Pharmacogenetics. 2001 Nov;11(8):663-9. (PMID: 11692074)
Biol Blood Marrow Transplant. 2009 Feb;15(2):231-41. (PMID: 19167683)
Biol Blood Marrow Transplant. 2008 Jan;14(1):88-98. (PMID: 18158965)
J Natl Cancer Inst. 1998 Apr 1;90(7):512-8. (PMID: 9539246)
Nucleic Acids Res. 2002 Jun 15;30(12):e57. (PMID: 12060695)
Appl Environ Microbiol. 1998 Oct;64(10):3724-30. (PMID: 9758791)
Bone Marrow Transplant. 2001 Dec;28(11):1013-8. (PMID: 11781609)
Bone Marrow Transplant. 2010 Feb;45(2):261-7. (PMID: 19584821)
Carcinogenesis. 1997 Apr;18(4):641-4. (PMID: 9111193)
Pharmacology. 2000 Sep;61(3):154-66. (PMID: 10971201)
Bone Marrow Transplant. 2006 Feb;37(4):345-51. (PMID: 16400337)
Pharmacogenetics. 2000 Aug;10(6):557-65. (PMID: 10975610)
Drug Metab Dispos. 1996 Sep;24(9):1015-9. (PMID: 8886613)
Biol Blood Marrow Transplant. 2011 Aug;17(8):1222-30. (PMID: 21215809)
Anticancer Res. 2007 Nov-Dec;27(6C):4377-80. (PMID: 18214047)
Bone Marrow Transplant. 2004 May;33(10):979-87. (PMID: 15064687)
Biochem Biophys Res Commun. 1997 Jun 18;235(2):424-8. (PMID: 9199210)
Bone Marrow Transplant. 2000 May;25(9):925-30. (PMID: 10800058)
Fundam Clin Pharmacol. 2008 Dec;22(6):605-8. (PMID: 19049662)
Expert Opin Drug Metab Toxicol. 2010 Feb;6(2):153-70. (PMID: 20078251)
Clin Chim Acta. 2006 Jun;368(1-2):93-8. (PMID: 16448639)
Blood. 2002 Oct 15;100(8):2703-7. (PMID: 12351375)
Bone Marrow Transplant. 2002 Aug;30(3):167-73. (PMID: 12189535)
المشرفين على المادة: EC 2.5.1.18 (Glutathione Transferase)
تواريخ الأحداث: Date Created: 20111104 Date Completed: 20120529 Latest Revision: 20211020
رمز التحديث: 20221213
DOI: 10.2165/11592520-000000000-00000
PMID: 22047155
قاعدة البيانات: MEDLINE
الوصف
تدمد:1179-2000
DOI:10.2165/11592520-000000000-00000