دورية أكاديمية
Serum low-molecular-weight protein fractionation for biomarker discovery.
| العنوان: | Serum low-molecular-weight protein fractionation for biomarker discovery. |
|---|---|
| المؤلفون: | VanMeter AJ; Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, VA, USA. avanmete@gmu.edu, Camerini S, Polci ML, Tessitore A, Trivedi N, Heiby M, Kamal Y, Hansen J, Zhou W |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2012; Vol. 823, pp. 237-49. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Blood Proteins/*analysis , Blood Proteins/*chemistry , Chemical Fractionation/*methods , Proteomics/*methods, Biomarkers/blood ; Blotting, Western ; Chromatography, Liquid ; Humans ; Mass Spectrometry ; Molecular Weight ; Protein Array Analysis |
| مستخلص: | Protein biomarkers provide the key diagnostic information for the detection of disease, risk of disease progression, and a patient's likely response to drug therapy. Potential biomarkers exist in biofluids, such as serum, urine, and cerebrospinal fluid. Unfortunately, discovering and validating protein biomarkers are hindered by the presence of high-molecular-weight proteins, such as serum albumin and immunoglobulins, which comprise 90% of the proteins present in these samples. High-abundance, high-molecular-weight proteins mask the low-molecular-weight (LMW) proteins and peptides using conventional protein detection methods. Candidate biomarkers are believed to exist in very low concentrations and comprise less than 1% of serum proteins, and may be highly labile as well. Therefore, it is imperative to isolate and enrich LMW proteins from complex mixtures for biomarker discovery. This chapter describes a continuous -elution electrophoresis method, based on molecular weight sieving, to isolate specific molecular weight fractions for mass spectrometric, western blotting, or protein array analysis. |
| المشرفين على المادة: | 0 (Biomarkers) 0 (Blood Proteins) |
| تواريخ الأحداث: | Date Created: 20111115 Date Completed: 20120427 Latest Revision: 20151119 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/978-1-60327-216-2_15 |
| PMID: | 22081349 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
|---|---|
| DOI: | 10.1007/978-1-60327-216-2_15 |