دورية أكاديمية
Expression changes of sex hormone binding globulin in GDM placental tissues.
| العنوان: | Expression changes of sex hormone binding globulin in GDM placental tissues. |
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| المؤلفون: | Sun L; Department of Obstetrics and Gynecology, Shengjing Hospital Affi liated to China Medical University, 36 Sanhao Street, Heping District, Shenyang, China., Jin Z, Teng W, Chi X, Zhang Y, Ai W, Wang P |
| المصدر: | Journal of perinatal medicine [J Perinat Med] 2011 Nov 19; Vol. 40 (2), pp. 129-35. Date of Electronic Publication: 2011 Nov 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Walter De Gruyter Country of Publication: Germany NLM ID: 0361031 Publication Model: Electronic Cited Medium: Internet ISSN: 1619-3997 (Electronic) Linking ISSN: 03005577 NLM ISO Abbreviation: J Perinat Med Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Berlin : Walter De Gruyter Original Publication: Berlin, New York, Walter de Gruyter. |
| مواضيع طبية MeSH: | Diabetes, Gestational/*metabolism , Placenta/*metabolism , Sex Hormone-Binding Globulin/*genetics, Adult ; Blotting, Western ; Diabetes, Gestational/pathology ; Female ; Gene Expression ; Gestational Age ; Humans ; Immunohistochemistry ; Microscopy, Immunoelectron ; Placenta/chemistry ; Placenta/ultrastructure ; Pregnancy ; RNA, Messenger/analysis ; Reverse Transcriptase Polymerase Chain Reaction ; Sex Hormone-Binding Globulin/analysis |
| مستخلص: | Objective: To compare the expression of sex hormone binding globulin (SHBG) in normal placental tissues with placental tissues from patients with gestational diabetes mellitus (GDM) and to deduce the mechanism affecting placental SHBG in GDM. Methods: We detected SHBG localization and measured SHBG mRNA and protein using immunohistochemistry, reverse-transcription polymerase chain reaction, and Western blotting, respectively, in normal and GDM placental tissues. The distribution of SHBG in placental cells was examined using immune electron microscopy. Results: Compared to controls, placental tissues from patients in the GDM group displayed disordered cell surface microvilli that were decreased in quantity, swollen, and had narrowed and broken gap junctions. Intracellular abnormalities included expanded rough endoplasmic reticula, swollen mitochondria, and irregular nuclear morphologies with non-uniform chromatin. SHBG localized primarily to trophoblast cell membranes and cytoplasm. SHBG was strongly expressed on the microvilli side and weakly expressed on the basement membrane with uneven staining. SHBG also was expressed in villous stromal cells and vascular endothelial cells. Compared to the controls, placental tissues from the GDM group displayed significantly decreased immunostaining rates for SHBG, as well as significantly lower levels of SHBG mRNA and protein expression (P<0.05). Conclusion: SHBG was detected in placental trophoblast cells from patients with GDM, and the synthesis and secretion of SHBG were reduced when trophoblast cells were irregular. A decrease in SHBG could affect placental function or aggravate GDM. Our results suggest that placental SHBG plays an important role in the pathogenesis of GDM. |
| المشرفين على المادة: | 0 (RNA, Messenger) 0 (Sex Hormone-Binding Globulin) |
| تواريخ الأحداث: | Date Created: 20111122 Date Completed: 20121022 Latest Revision: 20121107 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1515/JPM.2011.128 |
| PMID: | 22098307 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1619-3997 |
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| DOI: | 10.1515/JPM.2011.128 |