دورية أكاديمية
Altered plasma adipokine levels and in vitro adipocyte differentiation in pediatric type 1 diabetes.
| العنوان: | Altered plasma adipokine levels and in vitro adipocyte differentiation in pediatric type 1 diabetes. |
|---|---|
| المؤلفون: | Verrijn Stuart AA; Department of Paediatric Endocrinology, University Medical Center Utrecht, 3508 AB Utrecht, the Netherlands. a.a.verrijnstuart@umcutrecht.nl, Schipper HS, Tasdelen I, Egan DA, Prakken BJ, Kalkhoven E, de Jager W |
| المصدر: | The Journal of clinical endocrinology and metabolism [J Clin Endocrinol Metab] 2012 Feb; Vol. 97 (2), pp. 463-72. Date of Electronic Publication: 2011 Nov 23. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 0375362 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1945-7197 (Electronic) Linking ISSN: 0021972X NLM ISO Abbreviation: J Clin Endocrinol Metab Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2017- : New York : Oxford University Press Original Publication: Springfield, Ill. : Charles C. Thomas |
| مواضيع طبية MeSH: | Cell Differentiation*/drug effects, Adipocytes/*physiology , Adipokines/*blood , Diabetes Mellitus, Type 1/*blood , Diabetes Mellitus, Type 1/*physiopathology, Adipocytes/pathology ; Adolescent ; Cells, Cultured ; Child ; Cohort Studies ; Culture Media, Conditioned/pharmacology ; Cytokines/blood ; Diabetes Mellitus, Type 1/complications ; Diabetes Mellitus, Type 1/pathology ; Fatty Acids, Nonesterified/blood ; Fatty Acids, Nonesterified/physiology ; Female ; Humans ; Hyperglycemia/blood ; Hyperglycemia/complications ; Hyperglycemia/pathology ; Hyperglycemia/physiopathology ; Male ; Primary Cell Culture |
| مستخلص: | Context: Type 1 diabetes (T1D) is considered a proinflammatory condition. Adipose tissue involvement seems evident because adiponectin levels correlate with disease remission and administration of leptin suppresses the low-grade systemic inflammation in mice with T1D. Whether adipose tissue involvement in T1D already occurs at a young age is yet unknown. Objective: The aim was to explore the extent of adipokine alterations in pediatric T1D and gain more insight into the mechanisms underlying the involvement of adipose tissue. Design and Participants: First, plasma adipokine profiling (24 adipokines) of 20 children with onset T1D, 20 children with long-standing T1D, and 17 healthy controls was performed using a recently developed and validated multiplex immunoassay. Second, the effects of diabetic plasma factors on preadipocyte proliferation and differentiation were studied in vitro. Results: In children with onset and long-standing T1D, plasma adipokine profiling showed increased levels of various adipokines acting at the crossroads of adipose tissue function and inflammation, including CCL2/monocyte chemoattractant protein-1 and the novel adipokines cathepsin S, chemerin, and tissue inhibitor of metalloproteinase-1 (P < 0.05). Furthermore, onset and long-standing diabetic plasma significantly induced preadipocyte proliferation and adipocyte differentiation in vitro (P < 0.05). Two candidate plasma factors, glucose and the saturated fatty acid palmitic acid, did not affect proliferation or adipocyte differentiation in vitro but were found to increase CCL2 (monocyte chemoattractant protein-1) secretion by adipocytes. Conclusions: The adipogenic effects of diabetic plasma in vitro and the altered adipokine levels in vivo suggest adipose tissue involvement in the low-grade inflammation associated with T1D, already in pediatric patients. |
| المشرفين على المادة: | 0 (Adipokines) 0 (Culture Media, Conditioned) 0 (Cytokines) 0 (Fatty Acids, Nonesterified) |
| تواريخ الأحداث: | Date Created: 20111125 Date Completed: 20120409 Latest Revision: 20220331 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1210/jc.2011-1858 |
| PMID: | 22112811 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1945-7197 |
|---|---|
| DOI: | 10.1210/jc.2011-1858 |