دورية أكاديمية
أعرض في EDS

MicroRNA alterations and associated aberrant DNA methylation patterns across multiple sample types in oral squamous cell carcinoma.

التفاصيل البيبلوغرافية
العنوان: MicroRNA alterations and associated aberrant DNA methylation patterns across multiple sample types in oral squamous cell carcinoma.
المؤلفون: Wiklund ED; Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark., Gao S, Hulf T, Sibbritt T, Nair S, Costea DE, Villadsen SB, Bakholdt V, Bramsen JB, Sørensen JA, Krogdahl A, Clark SJ, Kjems J
المصدر: PloS one [PLoS One] 2011; Vol. 6 (11), pp. e27840. Date of Electronic Publication: 2011 Nov 22.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Carcinoma, Squamous Cell/*genetics , DNA Methylation/*genetics , MicroRNAs/*genetics , Mouth Neoplasms/*genetics, Aged ; Aged, 80 and over ; Cluster Analysis ; Epigenesis, Genetic ; Female ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Health ; Humans ; Male ; MicroRNAs/metabolism ; Middle Aged ; Neoplastic Stem Cells/metabolism ; Neoplastic Stem Cells/pathology ; Saliva/metabolism
مستخلص: Background: MicroRNA (miRNA) expression is broadly altered in cancer, but few studies have investigated miRNA deregulation in oral squamous cell carcinoma (OSCC). Epigenetic mechanisms are involved in the regulation of >30 miRNA genes in a range of tissues, and we aimed to investigate this further in OSCC.
Methods: TaqMan® qRT-PCR arrays and individual assays were used to profile miRNA expression in a panel of 25 tumors with matched adjacent tissues from patients with OSCC, and 8 control paired oral stroma and epithelium from healthy volunteers. Associated DNA methylation changes of candidate epigenetically deregulated miRNA genes were measured in the same samples using the MassArray® mass spectrometry platform. MiRNA expression and DNA methylation changes were also investigated in FACS sorted CD44(high) oral cancer stem cells from primary tumor samples (CSCs), and in oral rinse and saliva from 15 OSCC patients and 7 healthy volunteers.
Results: MiRNA expression patterns were consistent in healthy oral epithelium and stroma, but broadly altered in both tumor and adjacent tissue from OSCC patients. MiR-375 is repressed and miR-127 activated in OSCC, and we confirm previous reports of miR-137 hypermethylation in oral cancer. The miR-200 s/miR-205 were epigenetically activated in tumors vs normal tissues, but repressed in the absence of DNA hypermethylation specifically in CD44(high) oral CSCs. Aberrant miR-375 and miR-200a expression and miR-200c-141 methylation could be detected in and distinguish OSCC patient oral rinse and saliva from healthy volunteers, suggesting a potential clinical application for OSCC specific miRNA signatures in oral fluids.
Conclusions: MiRNA expression and DNA methylation changes are a common event in OSCC, and we suggest miR-375, miR-127, miR-137, the miR-200 family and miR-205 as promising candidates for future investigations. Although overall activated in OSCC, miR-200/miR-205 suppression in oral CSCs indicate that cell specific silencing of these miRNAs may drive tumor expansion and progression.
References: Clin Chem. 2010 Nov;56(11):1733-41. (PMID: 20847327)
Cell Cycle. 2009 Mar 15;8(6):843-52. (PMID: 19221491)
Carcinogenesis. 2010 Jan;31(1):27-36. (PMID: 19752007)
Biochem Biophys Res Commun. 2010 Apr 9;394(3):623-7. (PMID: 20226166)
Expert Rev Mol Diagn. 2010 Apr;10(3):297-308. (PMID: 20370587)
J Pathol. 2007 Dec;213(4):384-91. (PMID: 17935121)
Nat Cell Biol. 2008 May;10(5):593-601. (PMID: 18376396)
PLoS One. 2010 Jan 13;5(1):e8697. (PMID: 20084174)
Stat Appl Genet Mol Biol. 2004;3:Article3. (PMID: 16646809)
Biochem Biophys Res Commun. 2007 Jun 22;358(1):12-7. (PMID: 17475218)
Nat Med. 2011 Aug 07;17(9):1101-8. (PMID: 21822286)
Cancer Res. 2001 Apr 15;61(8):3225-9. (PMID: 11309270)
Cancer Res. 2001 Feb 1;61(3):939-42. (PMID: 11221887)
Cancer Res. 2007 Dec 15;67(24):11517-27. (PMID: 18089780)
CA Cancer J Clin. 1999 Jan-Feb;49(1):8-31, 1. (PMID: 10200775)
Cancer Res. 2010 Nov 15;70(22):9175-84. (PMID: 20978187)
Oral Oncol. 2008 Oct;44(10):910-4. (PMID: 18620891)
Oncogene. 2002 Jun 20;21(27):4231-6. (PMID: 12082610)
Epigenomics. 2010 Apr;2(2):245-69. (PMID: 22121873)
Cancer. 2011 Apr 1;117(7):1454-62. (PMID: 21425146)
Hum Mol Genet. 2009 Dec 15;18(24):4818-29. (PMID: 19776030)
Cancer Res. 2008 Apr 1;68(7):2094-105. (PMID: 18381414)
Trends Genet. 2000 Jun;16(6):276-7. (PMID: 10827456)
Mol Pathol. 2000 Aug;53(4):165-72. (PMID: 11040937)
EMBO J. 2011 Feb 16;30(4):770-82. (PMID: 21224848)
BMC Genomics. 2011 Jan 21;12:54. (PMID: 21255435)
Nucleic Acids Res. 2007;35(18):e119. (PMID: 17855397)
Nat Rev Cancer. 2006 Apr;6(4):259-69. (PMID: 16557279)
BMC Med. 2008 Jun 24;6:14. (PMID: 18577219)
J Oral Pathol Med. 2005 Feb;34(2):116-9. (PMID: 15641992)
J Pathol. 2011 Mar;223(4):482-95. (PMID: 21294122)
Carcinogenesis. 2010 May;31(5):864-70. (PMID: 20197299)
Cell. 2009 Aug 7;138(3):592-603. (PMID: 19665978)
Cell Cycle. 2007 May 2;6(9):1001-5. (PMID: 17457051)
FASEB J. 2010 Sep;24(9):3255-63. (PMID: 20439489)
Genes Dev. 2008 Apr 1;22(7):894-907. (PMID: 18381893)
Nat Cell Biol. 2009 Dec;11(12):1487-95. (PMID: 19935649)
Genes Chromosomes Cancer. 2009 Jul;48(7):569-82. (PMID: 19396866)
J Cell Biol. 2010 Apr 5;189(1):127-41. (PMID: 20368621)
Scand J Dent Res. 1979 Jun;87(3):234-43. (PMID: 293885)
Int J Cancer. 2001 Sep 1;93(5):667-73. (PMID: 11477576)
Nat Protoc. 2006;1(5):2353-64. (PMID: 17406479)
Cell Res. 2010 Jul;20(7):784-93. (PMID: 20548334)
Am J Pathol. 2009 Mar;174(3):736-45. (PMID: 19179615)
Anal Biochem. 2011 Oct 15;417(2):233-41. (PMID: 21741950)
Int J Cancer. 2004 Mar 20;109(2):230-7. (PMID: 14750174)
Int J Cancer. 2011 Mar 15;128(6):1327-34. (PMID: 20473948)
Cancer Res. 2010 Mar 15;70(6):2339-49. (PMID: 20215506)
Cancer Cell. 2006 Jun;9(6):435-43. (PMID: 16766263)
EMBO Rep. 2010 Sep;11(9):670-7. (PMID: 20706219)
Int J Cancer. 1993 Dec 2;55(6):891-903. (PMID: 8253525)
Cancer Res. 2007 Feb 15;67(4):1424-9. (PMID: 17308079)
RNA. 2008 May;14(5):844-52. (PMID: 18375788)
Cell. 2004 Jan 23;116(2):281-97. (PMID: 14744438)
المشرفين على المادة: 0 (MicroRNAs)
تواريخ الأحداث: Date Created: 20111202 Date Completed: 20120402 Latest Revision: 20211021
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC3222641
DOI: 10.1371/journal.pone.0027840
PMID: 22132151
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0027840