دورية أكاديمية
Mechanism and control of Genipa americana seed germination.
| العنوان: | Mechanism and control of Genipa americana seed germination. |
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| المؤلفون: | Queiroz SE; Instituto Federal Goiano, Campus Urutaí, Rodovia Geraldo Silva Nascimento Km 2.5, CEP 75790-000, Urutaí, Goiás, Brazil., da Silva EA, Davide AC, José AC, Silva AT, Fraiz AC, Faria JM, Hilhorst HW |
| المصدر: | Physiologia plantarum [Physiol Plant] 2012 Mar; Vol. 144 (3), pp. 263-76. Date of Electronic Publication: 2012 Jan 06. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Scandinavian Society For Plant Physiology Country of Publication: Denmark NLM ID: 1256322 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1399-3054 (Electronic) Linking ISSN: 00319317 NLM ISO Abbreviation: Physiol Plant Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Copenhagen : Scandinavian Society For Plant Physiology Original Publication: Lund, Sweden [etc.] |
| مواضيع طبية MeSH: | Germination*, Rubiaceae/*growth & development , Seeds/*growth & development, Abscisic Acid/pharmacology ; Amino Acid Sequence ; Biomechanical Phenomena ; Cell Wall/metabolism ; Cloning, Molecular ; DNA, Plant/genetics ; Enzyme Activation ; Enzyme Assays ; Fruit/growth & development ; Fruit/metabolism ; Gene Expression Regulation, Plant ; Genes, Plant ; Isoenzymes/genetics ; Isoenzymes/metabolism ; Microscopy, Electron, Scanning ; Molecular Sequence Data ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Rubiaceae/genetics ; Rubiaceae/metabolism ; Seeds/ultrastructure ; Sequence Alignment ; Water/metabolism ; beta-Mannosidase/metabolism |
| مستخلص: | Genipa americana (Rubiaceae) is important for restoration of riparian forest in the Brazilian Cerrado. The objective was to characterize the mechanism and control of germination of G. americana to support uniform seedling production. Morphology and morphometrics of seeds, embryo and endosperm were assessed by light and scanning electron microscopy during germination. Imbibition and germination curves were generated and over the same time interval endosperm digestion and resistance were measured by puncture force analysis and activity assay of endo-β-mannanase (EBM) in water and in abscisic acid (ABA). The gene encoding for EBM was partially cloned and its expression monitored by quantitative real-time-polymerase chain reaction. Embryos displayed growth prior to radicle protrusion. A two-phase increase in EBM activity coincided with the two stages of weakening of the micropylar endosperm. The second stage also coincided with growth of the embryo prior to radicle protrusion. Enzyme activity was initiated in the micropylar endosperm but spread to the lateral endosperm. ABA completely inhibited germination by inhibiting embryo growth, the second stage of weakening and expression of the EBM gene, but EBM activity was not significantly inhibited. This suggests that a specific isoform of the enzyme is involved in endosperm weakening. EBM may cause a general 'softening' of micropylar endosperm cell walls, allowing the embryo to puncture the endosperm as the driving force of the decrease in puncture force. (Copyright © Physiologia Plantarum 2011.) |
| المشرفين على المادة: | 0 (DNA, Plant) 0 (Isoenzymes) 0 (Plant Proteins) 059QF0KO0R (Water) 72S9A8J5GW (Abscisic Acid) EC 3.2.1.25 (beta-Mannosidase) |
| تواريخ الأحداث: | Date Created: 20111214 Date Completed: 20120521 Latest Revision: 20131121 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1111/j.1399-3054.2011.01554.x |
| PMID: | 22150432 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1399-3054 |
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| DOI: | 10.1111/j.1399-3054.2011.01554.x |