دورية أكاديمية
أعرض في EDS

Comparative gene expression study of the chronic exposure to clozapine and haloperidol in rat frontal cortex.

التفاصيل البيبلوغرافية
العنوان: Comparative gene expression study of the chronic exposure to clozapine and haloperidol in rat frontal cortex.
المؤلفون: Fatemi SH; Department of Psychiatry, Division of Neuroscience Research, University of Minnesota Medical School, 420 Delaware St. SE, MMC 392, Minneapolis, MN 55455, United States. fatem002@umn.edu, Folsom TD, Reutiman TJ, Novak J, Engel RH
المصدر: Schizophrenia research [Schizophr Res] 2012 Feb; Vol. 134 (2-3), pp. 211-8. Date of Electronic Publication: 2011 Dec 10.
نوع المنشور: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Publisher B. V Country of Publication: Netherlands NLM ID: 8804207 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-2509 (Electronic) Linking ISSN: 09209964 NLM ISO Abbreviation: Schizophr Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam : Elsevier Science Publisher B. V., c1988-
مواضيع طبية MeSH: Antipsychotic Agents/*pharmacology , Clozapine/*pharmacology , Frontal Lobe/*drug effects , Gene Expression Regulation/*drug effects , Haloperidol/*pharmacology, Animals ; Catechol O-Methyltransferase/genetics ; Catechol O-Methyltransferase/metabolism ; Cell Adhesion Molecules/genetics ; Cell Adhesion Molecules/metabolism ; Frontal Lobe/metabolism ; GPI-Linked Proteins/genetics ; GPI-Linked Proteins/metabolism ; Gene Expression Profiling ; Male ; RNA, Messenger/metabolism ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; bcl-X Protein/genetics ; bcl-X Protein/metabolism
مستخلص: Antipsychotic drugs (APDs) are effective in treating some of the positive and negative symptoms of schizophrenia. APDs take time to achieve a therapeutic effect which suggests that changes in gene expression are involved in their efficacy. We hypothesized that there would be altered expression of specific genes associated with the etiology or treatment of schizophrenia in frontal cortex of rats that received chronic treatment with a typical APD (haloperidol) vs. an atypical APD (clozapine). Rats were administered clozapine, haloperidol, or sterile saline intraperitoneally daily for 21days. Frontal cortices from clozapine-, haloperidol-, and saline-treated rats were dissected and subjected to microarray analysis. We observed a significant (1.5 fold, p<0.05) downregulation of 278 genes and upregulation of 73 genes in the clozapine-treated brains vs. controls and downregulation of 451 genes and upregulation of 115 genes in the haloperidol-treated brains vs. control. A total of 146 genes (130 downregulated and 16 upregulated) were significantly altered by both clozapine and haloperidol. These genes were classified by functional groups. qRT-PCR (quantitative real-time polymerase chain reaction) analysis verified the direction and magnitude of change for a group of nine genes significantly altered by clozapine and 11 genes significantly altered by haloperidol. Three genes verified by qRT-PCR were altered by both drugs: Bcl2-like 1 (Bcl2l1), catechol-O-methyltransferase (Comt), and opioid-binding protein/cell adhesion molecule-like (Opcml). Our results show that clozapine and haloperidol cause changes in levels of many important genes that may be involved in etiology and treatment of schizophrenia.
(Copyright © 2011 Elsevier B.V. All rights reserved.)
المشرفين على المادة: 0 (Antipsychotic Agents)
0 (Bcl2l1 protein, rat)
0 (Cell Adhesion Molecules)
0 (GPI-Linked Proteins)
0 (Opcml protein, rat)
0 (RNA, Messenger)
0 (bcl-X Protein)
EC 2.1.1.6 (Catechol O-Methyltransferase)
J60AR2IKIC (Clozapine)
J6292F8L3D (Haloperidol)
تواريخ الأحداث: Date Created: 20111214 Date Completed: 20120604 Latest Revision: 20220321
رمز التحديث: 20240628
DOI: 10.1016/j.schres.2011.11.013
PMID: 22154595
قاعدة البيانات: MEDLINE
الوصف
تدمد:1573-2509
DOI:10.1016/j.schres.2011.11.013