دورية أكاديمية
Effects of natural eggshell membrane (NEM) on cytokine production in cultures of peripheral blood mononuclear cells: increased suppression of tumor necrosis factor-α levels after in vitro digestion.
| العنوان: | Effects of natural eggshell membrane (NEM) on cytokine production in cultures of peripheral blood mononuclear cells: increased suppression of tumor necrosis factor-α levels after in vitro digestion. |
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| المؤلفون: | Benson KF; NIS Labs , Klamath Falls, Oregon, USA., Ruff KJ, Jensen GS |
| المصدر: | Journal of medicinal food [J Med Food] 2012 Apr; Vol. 15 (4), pp. 360-8. Date of Electronic Publication: 2011 Dec 14. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 9812512 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-7600 (Electronic) Linking ISSN: 1096620X NLM ISO Abbreviation: J Med Food Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., c1998- |
| مواضيع طبية MeSH: | Anti-Inflammatory Agents/*pharmacology , Egg Shell/*chemistry , Immunologic Factors/*biosynthesis , Leukocytes, Mononuclear/*drug effects , Leukocytes, Mononuclear/*metabolism , Tumor Necrosis Factor-alpha/*antagonists & inhibitors, Adult ; Animals ; Cell Proliferation ; Cells, Cultured ; Electrophoresis, Polyacrylamide Gel ; Humans ; Interferon-gamma/biosynthesis ; Interleukin-10/biosynthesis ; Interleukin-2/biosynthesis ; Interleukin-4/biosynthesis ; Interleukin-6/biosynthesis ; Middle Aged ; Phytohemagglutinins/pharmacology ; Tumor Necrosis Factor-alpha/blood ; Young Adult |
| مستخلص: | Tumor necrosis factor-α (TNF-α) plays an important role in inflammatory processes. This study examined the effects of natural eggshell membrane (NEM(®)) (ESM Technologies, LLC, Carthage, MO, USA) on interleukin (IL)-2, IL-4, IL-6, IL-10, interferon-γ (IFN-γ), and TNF-α cytokine production by 4-day peripheral blood mononuclear cell (PBMC) cultures exposed to serial dilutions of either an aqueous extract of natural eggshell membrane (NEM-AQ) or NEM subjected to in vitro digestion (NEM-IVD). The effects on cytokine production were also assessed in the presence of phytohemagglutinin (PHA) and pokeweed mitogen (PWM) where exposure to NEM-AQ resulted in reduced levels of proliferation and statistically significant effects on IL-6, IL-10, IFN-γ, and TNF-α cytokine production. NEM-AQ reduced levels of IL-6, IL-10, IFN-γ, and TNF-α in cultures exposed to PHA. In cultures containing PWM, NEM-AQ reduced production of IL-10 and at the highest dose tested increased IL-6 and decreased TNF-α cytokine levels. NEM-IVD, at the two lowest concentrations of product, significantly reduced TNF-α production by PBMC cultures exposed to PWM compared with the in vitro digest control or native NEM. Taken together, these results suggest that NEM-AQ can influence signaling events in response to the T cell-specific mitogen PHA as well as to the mitogen PWM that require cellular cross-talk and that these effects may be partially mediated through a reduction in level of the pro-inflammatory cytokine TNF-α. The suppression of TNF-α production in the presence of NEM-IVD is promising for the use of NEM as a consumable anti-inflammatory product. |
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| المشرفين على المادة: | 0 (Anti-Inflammatory Agents) 0 (Immunologic Factors) 0 (Interleukin-2) 0 (Interleukin-6) 0 (Phytohemagglutinins) 0 (Tumor Necrosis Factor-alpha) 130068-27-8 (Interleukin-10) 207137-56-2 (Interleukin-4) 82115-62-6 (Interferon-gamma) |
| تواريخ الأحداث: | Date Created: 20111216 Date Completed: 20120716 Latest Revision: 20220318 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC3308710 |
| DOI: | 10.1089/jmf.2011.0197 |
| PMID: | 22168811 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1557-7600 |
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| DOI: | 10.1089/jmf.2011.0197 |