دورية أكاديمية
أعرض في EDS

Erythropoietin attenuates apoptosis after ischemia-reperfusion-induced renal injury in transiently hyperglycemic Wister rats.

التفاصيل البيبلوغرافية
العنوان: Erythropoietin attenuates apoptosis after ischemia-reperfusion-induced renal injury in transiently hyperglycemic Wister rats.
المؤلفون: Caetano AM; Department of Anesthesilogy, São Paulo State University UNESP, Botucatu, São Paulo, Brazil., Vianna Filho PT, Castiglia YM, Golim MA, de Souza AV, de Carvalho LR, Deffune E, de Oliveira C, Vianna PT
المصدر: Transplantation proceedings [Transplant Proc] 2011 Dec; Vol. 43 (10), pp. 3618-21.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Inc Country of Publication: United States NLM ID: 0243532 Publication Model: Print Cited Medium: Internet ISSN: 1873-2623 (Electronic) Linking ISSN: 00411345 NLM ISO Abbreviation: Transplant Proc Subsets: MEDLINE
أسماء مطبوعة: Publication: New York, N.Y. : Elsevier Science Inc.
Original Publication: New York Stratton.
مواضيع طبية MeSH: Apoptosis/*drug effects , Erythropoietin/*pharmacology , Hyperglycemia/*complications , Kidney/*drug effects , Kidney/*surgery , Nephrectomy/*adverse effects , Reperfusion Injury/*prevention & control, Animals ; Blood Glucose/metabolism ; Creatinine/blood ; Cytoprotection ; Disease Models, Animal ; Epoetin Alfa ; Flow Cytometry ; Kidney/blood supply ; Kidney/pathology ; Kidney Glomerulus/drug effects ; Kidney Glomerulus/pathology ; Kidney Tubules/drug effects ; Kidney Tubules/pathology ; Male ; Necrosis ; Rats ; Rats, Wistar ; Recombinant Proteins/pharmacology ; Reperfusion Injury/etiology ; Reperfusion Injury/pathology ; Time Factors
مستخلص: Background: Hyperglycemia is associated with a decreased tolerance to ischemia and an increased severity of renal ischemia reperfusion (I/R) injury. It has been suggested that erythropoietin (EPO) attenuates this effect in normoglycemic animals. This study sought to examine the effects of EPO on treatment renal I/R injury (IRI) in transiently hyperglycemic rats.
Material and Methods: Twenty-eight male Wister rats anesthetized with isoflurane received glucose (2.5 g.kg(-1) intraperitoneally) before right nephrectomy. They were randomly assigned to four groups: sham operation (S); IRI (ISO); IRI+EPO, (600 UI kg(-1) low-dose EPO [EL]); and IRI+EPO 5000 UI kg(-1) (high-dose EPO [EH]). IRI was induced by a 25-minute period of left renal ischemia followed by reperfusion for 24 hours. Serum creatinine and glucose levels were measure at baseline (M1), immediately after the ischemic period (M2), and at 24 hours after reperfusion (M3). After sacrificing the animals, left kidney specimens were submitted for histological analysis including flow cytometry to estimate tubular necrosis and the percentages of apoptotic, dead or intact cells.
Results: Scr in the ISO group was significantly higher at M3 than among the other groups. Percentages of early apoptotic cells in ISO group were significantly higher than the other groups. Percentages of late apoptotic cells in S and ISO groups were significantly greater than EL and EH groups. However, no significant intergroup differences were observed regarding the incidence of tubular necrosis.
Conclusions: Our results suggested that, although not preventing the occurrence of tubular necrosis, EPO attenuated apoptosis and glomerular functional impairment among transiently hyperglycemic rats undergoing an ischemia/reperfusion insult.
(Copyright © 2011 Elsevier Inc. All rights reserved.)
المشرفين على المادة: 0 (Blood Glucose)
0 (Recombinant Proteins)
11096-26-7 (Erythropoietin)
64FS3BFH5W (Epoetin Alfa)
AYI8EX34EU (Creatinine)
تواريخ الأحداث: Date Created: 20111217 Date Completed: 20120329 Latest Revision: 20220330
رمز التحديث: 20221213
DOI: 10.1016/j.transproceed.2011.10.049
PMID: 22172815
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-2623
DOI:10.1016/j.transproceed.2011.10.049