دورية أكاديمية
Direct fluorescent antibody assay and polymerase chain reaction for the detection of Chlamydia trachomatis in patients with vernal keratoconjunctivitis.
| العنوان: | Direct fluorescent antibody assay and polymerase chain reaction for the detection of Chlamydia trachomatis in patients with vernal keratoconjunctivitis. |
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| المؤلفون: | Nishiwaki-Dantas MC; Department of Ophthalmology, Corneal and External Disease Service, Santa Casa of São Paulo, Brazil., de Abreu MT, de Melo CM, Romero IL, Neto RB, Dantas PE |
| المصدر: | Clinics (Sao Paulo, Brazil) [Clinics (Sao Paulo)] 2011; Vol. 66 (12), pp. 2013-8. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 101244734 Publication Model: Print Cited Medium: Internet ISSN: 1980-5322 (Electronic) Linking ISSN: 18075932 NLM ISO Abbreviation: Clinics (Sao Paulo) Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2022- : [New York] : Elsevier Original Publication: São Paulo, SP : Hospital das Clínicas, Faculty of Medicine, University of São Paulo, [2005]- |
| مواضيع طبية MeSH: | Chlamydia trachomatis*/genetics , Chlamydia trachomatis*/immunology, Chlamydia Infections/*diagnosis , Conjunctivitis, Allergic/*diagnosis, Adolescent ; Adult ; Case-Control Studies ; Child ; Child, Preschool ; Chronic Disease ; Conjunctivitis, Allergic/microbiology ; Female ; Fluorescent Antibody Technique, Direct ; Humans ; Male ; Polymerase Chain Reaction ; ROC Curve ; Sensitivity and Specificity ; Young Adult |
| مستخلص: | Objectives: To identify Chlamydia trachomatis via polymerase chain reaction and a direct fluorescent antibody assay in patients with vernal keratoconjunctivitis while comparing the efficacies of both tests for detecting Chlamydia trachomatis in these conditions. Methods: Conjunctival scraping samples were obtained from 177 patients who were divided into two groups: a vernal keratoconjunctivitis group (group A) and a control group (group B). The polymerase chain reaction and a direct fluorescent antibody assay were performed. Sensitivity, specificity, receiver operating characteristic curves, and areas under the curve were calculated for both tests in groups A and B. Receiver operating characteristic curves were plotted using a categorical variable with only two possible outcomes (positive and negative). Results: Statistical analysis revealed a significant association between vernal keratoconjunctivitis and Chlamydia trachomatis infection detected by a direct fluorescent antibody assay with high sensitivity and specificity. All patients in group A with positive polymerase chain reactions also presented with positive direct fluorescent antibody assays. Conclusion: The association between vernal keratoconjunctivitis and Chlamydia trachomatis infection was confirmed by positive direct fluorescent antibody assays in 49.4% of vernal keratoconjunctivitis patients and by positive polymerase chain reactions in 20% of these patients. The direct fluorescent antibody assay detected Chlamydia trachomatis in a higher number of patients than did the polymerase chain reaction. Although the diagnosis of trachoma is essentially clinical, the disease may not be detected in vernal keratoconjunctivitis patients. Due to the high frequency of chlamydial infection detected in patients with vernal keratoconjunctivitis, we suggest considering routine laboratory tests to detect Chlamydia trachomatis in patients with severe and refractory allergic disease. |
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| تواريخ الأحداث: | Date Created: 20111223 Date Completed: 20120419 Latest Revision: 20220629 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC3226593 |
| DOI: | 10.1590/s1807-59322011001200003 |
| PMID: | 22189723 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1980-5322 |
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| DOI: | 10.1590/s1807-59322011001200003 |