دورية أكاديمية
In vitro monitoring of i-NOS concentrations with an immunosensor: the inhibitory effect of endocrine disruptors on i-NOS release.
| العنوان: | In vitro monitoring of i-NOS concentrations with an immunosensor: the inhibitory effect of endocrine disruptors on i-NOS release. |
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| المؤلفون: | Chandra P; Department of Chemistry and Institute of Innovative BioPhysio Sensor Technology, Pusan National University, Busan 609-735, South Korea., Koh WC, Noh HB, Shim YB |
| المصدر: | Biosensors & bioelectronics [Biosens Bioelectron] 2012 Feb 15; Vol. 32 (1), pp. 278-82. Date of Electronic Publication: 2011 Dec 09. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Advanced Technology Country of Publication: England NLM ID: 9001289 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4235 (Electronic) Linking ISSN: 09565663 NLM ISO Abbreviation: Biosens Bioelectron Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Elsevier Advanced Technology Original Publication: [Barking, Essex, England] : Elsevier Applied Science, 1989- |
| مواضيع طبية MeSH: | Biosensing Techniques*/methods, Endocrine Disruptors/*pharmacology , Neurons/*enzymology , Nitric Oxide Synthase Type II/*analysis , Nitric Oxide Synthase Type II/*antagonists & inhibitors , Phenols/*pharmacology, Animals ; Antibodies, Immobilized/immunology ; Benzhydryl Compounds ; Cell Line ; Electrochemical Techniques/methods ; Gold/chemistry ; Humans ; Immunoassay/methods ; Nanoparticles/chemistry ; Neurons/drug effects ; Nitric Oxide Synthase Type II/immunology |
| مستخلص: | The amperometric immunosensor has demonstrated the toxicity of endocrine disrupters (EDs) through monitoring the in vitro i-NOS concentration change, where the antibody of inducible nitric oxide synthase (i-NOS) was immobilized on the conducting polymer-gold nanoparticles composite. The performance of the sensor and the experimental parameters affecting the immunoreaction were optimized. Neuronal cells treated by EDs decreased in the in vitro i-NOS concentration. The effect of bisphenol A (BPA) on the i-NOS concentration released in the cells was investigated with different incubation times, and the interfering by nonspecific binding species present in a neuronal cell lysate was also examined. Of all the tested EDs, BPA showed the inhibitoriest effect and the minimum inhibitory concentration of BPA affecting the i-NOS concentration was 0.09 ± 0.005 μM. The result shows that monitoring of i-NOS in the neuronal cells treated by EDs will be a useful method to evaluate the toxic behavior of EDs. (Copyright © 2011 Elsevier B.V. All rights reserved.) |
| المشرفين على المادة: | 0 (Antibodies, Immobilized) 0 (Benzhydryl Compounds) 0 (Endocrine Disruptors) 0 (Phenols) 7440-57-5 (Gold) EC 1.14.13.39 (Nitric Oxide Synthase Type II) MLT3645I99 (bisphenol A) |
| تواريخ الأحداث: | Date Created: 20111227 Date Completed: 20120514 Latest Revision: 20131121 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.bios.2011.11.027 |
| PMID: | 22196875 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-4235 |
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| DOI: | 10.1016/j.bios.2011.11.027 |