دورية أكاديمية
Trypanocide, cytotoxic, and antifungal activities of Momordica charantia.
| العنوان: | Trypanocide, cytotoxic, and antifungal activities of Momordica charantia. |
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| المؤلفون: | Santos KK; Laboratório de Microbiologia e Biologia Molecular, Universidade Regional do Cariri, Crato (CE), Brasil., Matias EF, Sobral-Souza CE, Tintino SR, Morais-Braga MF, Guedes GM, Santos FA, Sousa AC, Rolón M, Vega C, de Arias AR, Costa JG, Menezes IR, Coutinho HD |
| المصدر: | Pharmaceutical biology [Pharm Biol] 2012 Feb; Vol. 50 (2), pp. 162-6. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 9812552 Publication Model: Print Cited Medium: Internet ISSN: 1744-5116 (Electronic) Linking ISSN: 13880209 NLM ISO Abbreviation: Pharm Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: [London] : Taylor & Francis Original Publication: Lisse, the Netherlands : Swets & Zeitlinger, c1998- |
| مواضيع طبية MeSH: | Antifungal Agents/*pharmacology , Momordica charantia/*chemistry , Plant Extracts/*pharmacology , Trypanocidal Agents/*pharmacology, Animals ; Antifungal Agents/isolation & purification ; Antifungal Agents/toxicity ; Candida/drug effects ; Cell Line ; Drug Synergism ; Inhibitory Concentration 50 ; Macrophages/drug effects ; Macrophages/metabolism ; Medicine, Traditional ; Metronidazole/administration & dosage ; Metronidazole/pharmacology ; Mice ; Microbial Sensitivity Tests ; Parasitic Sensitivity Tests ; Plant Extracts/administration & dosage ; Plant Extracts/toxicity ; Plant Leaves ; Toxicity Tests ; Trypanocidal Agents/isolation & purification ; Trypanocidal Agents/toxicity ; Trypanosoma cruzi/drug effects |
| مستخلص: | Context: Chagas disease, caused by Trypanosoma cruzi, is a public health problem. Currently, chemotherapy is the only available treatment for this disease, and the drugs used, nifurtimox and benzonidazol, present high toxicity levels. An alternative for replacing these drugs are natural extracts from Momordica charantia L. (Cucurbitaceae) used in traditional medicine because of their antimicrobial and biological activities. Objective: In this study, we evaluated the extract of M. charantia for its antiepimastigote, antifungal, and cytotoxic activities. Materials and Methods: An ethanol extract of leaves from M. charantia was prepared. To research in vitro antiepimastigote activity, T. cruzi CL-B5 clone was used. Epimastigotes were inoculated at a concentration of 1 × 10(5) cells/mL in 200 µl tryptose-liver infusion. For the cytotoxicity assay, J774 macrophages were used. The antifungal activity was evaluated by microdilution using strains of Candida albicans, Candida tropicalis, and Candida krusei. Results: The effective concentration capable of killing 50% of parasites (IC(50)) was 46.06 µg/mL. The minimum inhibitory concentration (MIC) was ≤ 1024 µg/mL. Metronidazole showed a potentiation of its antifungal effect when combined with an extract of M. charantia. Conclusions: Our results indicate that M. charantia could be a source of plant-derived natural products with antiepimastigote and antifungal-modifying activity with moderate toxicity. |
| المشرفين على المادة: | 0 (Antifungal Agents) 0 (Plant Extracts) 0 (Trypanocidal Agents) 140QMO216E (Metronidazole) |
| تواريخ الأحداث: | Date Created: 20120113 Date Completed: 20120504 Latest Revision: 20131121 |
| رمز التحديث: | 20221213 |
| DOI: | 10.3109/13880209.2011.581672 |
| PMID: | 22235885 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 1744-5116 |
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| DOI: | 10.3109/13880209.2011.581672 |