دورية أكاديمية
أعرض في EDS

Local administration of glucocorticoids decreases synovial citrullination in rheumatoid arthritis.

التفاصيل البيبلوغرافية
العنوان: Local administration of glucocorticoids decreases synovial citrullination in rheumatoid arthritis.
المؤلفون: Makrygiannakis D; Department of Medicine, Rheumatology Unit, Karolinska University Hospital, Karolinska Institutet, SE- 141 86, Stockholm, Sweden., Revu S, Engström M, af Klint E, Nicholas AP, Pruijn GJ, Catrina AI
المصدر: Arthritis research & therapy [Arthritis Res Ther] 2012 Jan 27; Vol. 14 (1), pp. R20. Date of Electronic Publication: 2012 Jan 27.
نوع المنشور: Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101154438 Publication Model: Electronic Cited Medium: Internet ISSN: 1478-6362 (Electronic) Linking ISSN: 14786354 NLM ISO Abbreviation: Arthritis Res Ther Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, 2003-
مواضيع طبية MeSH: Antirheumatic Agents/*therapeutic use , Arthritis, Rheumatoid/*drug therapy , Citrulline/*metabolism , Glucocorticoids/*therapeutic use , Synovial Membrane/*drug effects, Adult ; Aged ; Aged, 80 and over ; Antirheumatic Agents/administration & dosage ; Arthritis, Rheumatoid/metabolism ; Arthritis, Rheumatoid/pathology ; Dexamethasone/administration & dosage ; Dexamethasone/therapeutic use ; Double-Blind Method ; Female ; Glucocorticoids/administration & dosage ; Humans ; Hydrolases/metabolism ; Immunohistochemistry ; Injections, Intra-Articular ; Male ; Methotrexate/administration & dosage ; Methotrexate/therapeutic use ; Middle Aged ; Peptides/metabolism ; Protein-Arginine Deiminase Type 2 ; Protein-Arginine Deiminase Type 4 ; Protein-Arginine Deiminases ; Synovial Membrane/metabolism ; Synovial Membrane/pathology ; Time Factors ; Tissue Culture Techniques ; Treatment Outcome ; Triamcinolone Acetonide/administration & dosage ; Triamcinolone Acetonide/analogs & derivatives ; Triamcinolone Acetonide/therapeutic use
مستخلص: Introduction: Protein citrullination is present in the rheumatoid synovium, presumably contributing to the perpetuation of chronic inflammation, in the presence of specific autoimmunity. As a result, the present study examined the possibility that effective antirheumatic treatment will decrease the level of synovial citrullination.
Methods: Synovial biopsies were obtained from 11 rheumatoid arthritis (RA) patients before and after 8 weeks of treatment with 20 mg methotrexate weekly, 15 RA patients before and 2 weeks after an intraarticular glucocorticoid injection, and eight healthy volunteers. Synovial inflammation was assessed with double-blind semiquantitative analysis of lining thickness, cell infiltration, and vascularity by using a 4-point scale. Expression of citrullinated proteins (CPs) with the monoclonal antibody F95 and peptidylarginine deiminase (PAD) 2 and 4 was assessed immunohistochemically with double-blind semiquantitative analysis. In vitro synovial fluid (SF), peripheral blood (PB), mononuclear cells (MCs), and synovial explants obtained from RA patients were incubated with dexamethasone and analyzed with immunohistochemistry for expression of CP as well as PAD2 and PAD4 enzymes.
Results: The presence of synovial CP was almost exclusive in RA compared with healthy synovium and correlated with the degree of local inflammation. Treatment with glucocorticoids but not methotrexate alters expression of synovial CP and PAD enzymes, in parallel with a decrease of synovial inflammation. Ex vivo and in vitro studies suggest also a direct effect of glucocorticoids on citrullination, as demonstrated by the decrease in the level of citrullination and PAD expression after incubation of SFMC and synovial explants with dexamethasone.
Conclusion: Synovial citrullination and PAD expression are dependent on local inflammation and targeted by glucocorticoids.
التعليقات: Comment in: Arthritis Res Ther. 2012;14(1):108. (PMID: 22380578)
References: Exp Dermatol. 1999 Oct;8(5):392-401. (PMID: 10536966)
Ann Rheum Dis. 2008 Oct;67(10):1488-92. (PMID: 18413445)
Arthritis Rheum. 2004 Nov;50(11):3485-94. (PMID: 15529392)
Arthritis Rheum. 2009 Oct;60(10):2880-91. (PMID: 19790066)
Ann Rheum Dis. 2004 Apr;63(4):373-81. (PMID: 15020330)
Arthritis Rheum. 2005 Jan;52(1):61-72. (PMID: 15641091)
J Comp Neurol. 2005 Jun 6;486(3):243-53. (PMID: 15844172)
Arthritis Rheum. 2006 Jan;54(1):38-46. (PMID: 16385494)
Proteomics Clin Appl. 2010 May;4(5):511-8. (PMID: 21137068)
Glia. 2002 Mar 15;37(4):328-36. (PMID: 11870872)
Arthritis Rheum. 2000 Jan;43(1):155-63. (PMID: 10643712)
J Immunol. 2005 Apr 15;174(8):5057-64. (PMID: 15814737)
J Immunol. 2008 Feb 1;180(3):1895-902. (PMID: 18209087)
Annu Rev Immunol. 2008;26:651-75. (PMID: 18173373)
Arthritis Rheum. 2010 May;62(5):1213-23. (PMID: 20213805)
Arthritis Rheum. 2005 Aug;52(8):2323-30. (PMID: 16052592)
Osteoarthritis Cartilage. 1999 Jul;7(4):361-3. (PMID: 10419767)
Rheumatology (Oxford). 2005 Nov;44(11):1374-82. (PMID: 16105911)
Arch Biochem Biophys. 2002 Nov 1;407(1):25-31. (PMID: 12392711)
Arthritis Rheum. 1988 Mar;31(3):315-24. (PMID: 3358796)
Arthritis Rheum. 2007 Nov;56(11):3541-53. (PMID: 17968929)
Ann Rheum Dis. 2006 Sep;65(9):1219-22. (PMID: 16540548)
Anal Biochem. 1992 May 15;203(1):94-100. (PMID: 1524220)
J Comp Neurol. 2003 May 5;459(3):251-66. (PMID: 12655508)
Rheumatology (Oxford). 2005 Jan;44(1):40-50. (PMID: 15466895)
المشرفين على المادة: 0 (Antirheumatic Agents)
0 (Glucocorticoids)
0 (Peptides)
29VT07BGDA (Citrulline)
7S5I7G3JQL (Dexamethasone)
EC 3.- (Hydrolases)
EC 3.5.3.15 (PADI2 protein, human)
EC 3.5.3.15 (PADI4 protein, human)
EC 3.5.3.15 (Protein-Arginine Deiminase Type 2)
EC 3.5.3.15 (Protein-Arginine Deiminase Type 4)
EC 3.5.3.15 (Protein-Arginine Deiminases)
F446C597KA (Triamcinolone Acetonide)
I7GT1U99Y9 (triamcinolone hexacetonide)
YL5FZ2Y5U1 (Methotrexate)
تواريخ الأحداث: Date Created: 20120131 Date Completed: 20121217 Latest Revision: 20211021
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC3392813
DOI: 10.1186/ar3702
PMID: 22284820
قاعدة البيانات: MEDLINE
الوصف
تدمد:1478-6362
DOI:10.1186/ar3702