دورية أكاديمية
Comparison of in vitro and in vivo systems to study ica-independent Staphylococcus aureus biofilms.
| العنوان: | Comparison of in vitro and in vivo systems to study ica-independent Staphylococcus aureus biofilms. |
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| المؤلفون: | Ferreira FA; Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Departamento de Microbiologia Médica, Rio de Janeiro, RJ, Brazil., Souza RR, Bonelli RR, Américo MA, Fracalanzza SE, Figueiredo AM |
| المصدر: | Journal of microbiological methods [J Microbiol Methods] 2012 Mar; Vol. 88 (3), pp. 393-8. Date of Electronic Publication: 2012 Jan 24. |
| نوع المنشور: | Comparative Study; Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Biomedical Country of Publication: Netherlands NLM ID: 8306883 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8359 (Electronic) Linking ISSN: 01677012 NLM ISO Abbreviation: J Microbiol Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, The Netherlands : Elsevier Biomedical, c1983- |
| مواضيع طبية MeSH: | Biofilms/*growth & development , Fibronectins/*metabolism , Methicillin-Resistant Staphylococcus aureus/*growth & development, Animals ; Environmental Microbiology ; Foreign Bodies/microbiology ; Human Experimentation ; Humans ; Male ; Methicillin-Resistant Staphylococcus aureus/metabolism ; Mice ; Mice, Inbred BALB C ; Spectrum Analysis/methods ; Statistics as Topic |
| مستخلص: | The ability of Staphylococcus aureus to form biofilms is considered an important factor in the pathogenesis of central venous catheter-related bacteremia and infections associated with the use of medical prostheses. Different methods have been described for assessing staphylococcal biofilms, but few comparative studies have been attempted to evaluate these techniques; especially related to ica-independent biofilm formation/accumulation. In this study we compared some in vitro and in vivo techniques to evaluate ica-independent biofilms produced by methicillin-resistant S. aureus. We observed that biofilms formed on human fibronectin-covered surfaces were about three times higher than those produced on inert polystyrene surfaces. However, despite the difference in absolute values, a linear correlation was detected between these two models. We also found that biofilms formed on polystyrene or polyurethane surfaces treated with human serum were easily detachable during washing and staining processes. The mouse model of subcutaneous foreign body showed good correlation with the in vitro techniques using either inert polystyrene or solid-phase fibronectin. Thus, our data showed that the microtiter-plate-based spectrophotometric assay is an appropriate method for preliminary biofilm investigations, mainly when a large number of isolates, mutants or systems need to be tested. (Copyright © 2012 Elsevier B.V. All rights reserved.) |
| المشرفين على المادة: | 0 (Fibronectins) |
| تواريخ الأحداث: | Date Created: 20120203 Date Completed: 20120622 Latest Revision: 20191210 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.mimet.2012.01.007 |
| PMID: | 22296887 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1872-8359 |
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| DOI: | 10.1016/j.mimet.2012.01.007 |