دورية أكاديمية
Cellular characterisation of Candida tropicalis presenting fluconazole-related trailing growth.
| العنوان: | Cellular characterisation of Candida tropicalis presenting fluconazole-related trailing growth. |
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| المؤلفون: | Dornelas-Ribeiro M; Laboratório de Bacteriologia/Micologia, Instituto de Biologia do Exército, Rio de Janeiro, Brasil., Pinheiro EO, Guerra C, Braga-Silva LA, Carvalho SM, Santos AL, Rozental S, Fracalanzza SE |
| المصدر: | Memorias do Instituto Oswaldo Cruz [Mem Inst Oswaldo Cruz] 2012 Feb; Vol. 107 (1), pp. 31-8. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Instituto Oswaldo Cruz Country of Publication: Brazil NLM ID: 7502619 Publication Model: Print Cited Medium: Internet ISSN: 1678-8060 (Electronic) Linking ISSN: 00740276 NLM ISO Abbreviation: Mem Inst Oswaldo Cruz Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Rio de Janeiro : Instituto Oswaldo Cruz |
| مواضيع طبية MeSH: | Antifungal Agents/*pharmacology , Candida tropicalis/*drug effects , Fluconazole/*pharmacology, Candida tropicalis/growth & development ; Candida tropicalis/ultrastructure ; Humans ; Microbial Sensitivity Tests/methods ; Microscopy, Electron, Transmission ; Time Factors |
| مستخلص: | We assessed fluconazole susceptibility in 52 Candida tropicalis clinical strains using seven antifungal susceptibility methods, including broth microdilution (BMD) [standard M27 A3 (with neutral and acid pH), ATB Fungus 3, Vitek 2 system and flow cytometric analysis] and agar-based methods (disk diffusion and E-test). Trailing growth, detection of cell-associated secreted aspartic proteases (Saps) and morphological and ultrastructural traits of these clinical strains were also examined. The ranges of fluconazole 24 h-minimum inhibitory concentration (MIC) values were similar among all methods. The essential agreement among the methods used for MIC determinations was excellent and all methods categorised all strains as susceptible, except for one strain that showed a minor error. The presence of the trailing effect was assessed by six methods. Trailing positivity was observed for 86.5-100% of the strains. The exception was the BMD-Ac method where trailing growth was not observed. Morphological and ultrastructural alterations were detected in C. tropicalis trailing cells, including mitochondrial swelling and cell walls with irregular shapes. We tested the production of Saps in 13 C. tropicalis strains expressing trailing growth through flow cytometry. Our results showed that all of the C. tropicalis strains up-regulated surface Sap expression after 24 h or 48 h of exposure to fluconazole, which was not observed in untreated yeast strains. We concluded that C. tropicalis strains expressing trailing growth presented some particular features on both biological and ultrastructural levels. |
| المشرفين على المادة: | 0 (Antifungal Agents) 8VZV102JFY (Fluconazole) |
| تواريخ الأحداث: | Date Created: 20120208 Date Completed: 20120928 Latest Revision: 20190606 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1590/s0074-02762012000100005 |
| PMID: | 22310533 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1678-8060 |
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| DOI: | 10.1590/s0074-02762012000100005 |