دورية أكاديمية
Methylation of secreted frizzled related protein gene in acute leukemia patients in China.
| العنوان: | Methylation of secreted frizzled related protein gene in acute leukemia patients in China. |
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| المؤلفون: | Shen JZ; Department of Hematology, Union Hospital of Fujian Medical University, Fujian Institute of Hematology, Fuzhou, China. jianzhen_shen@126.com, Xu CB, Fu HY, Wu DS, Zhou HR, Fan LP |
| المصدر: | Asian Pacific journal of cancer prevention : APJCP [Asian Pac J Cancer Prev] 2011; Vol. 12 (10), pp. 2617-21. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Asian Pacific Organization for Cancer Prevention Country of Publication: Thailand NLM ID: 101130625 Publication Model: Print Cited Medium: Internet ISSN: 2476-762X (Electronic) Linking ISSN: 15137368 NLM ISO Abbreviation: Asian Pac J Cancer Prev Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bangkok : Asian Pacific Organization for Cancer Prevention, |
| مواضيع طبية MeSH: | DNA Methylation*, CpG Islands/*genetics , Frizzled Receptors/*genetics , Frizzled Receptors/*metabolism , Leukemia/*genetics, Adaptor Proteins, Signal Transducing ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Azacitidine/analogs & derivatives ; Azacitidine/pharmacology ; Cell Line, Tumor ; China ; DNA (Cytosine-5-)-Methyltransferases/metabolism ; DNA Methyltransferase 3A ; Decitabine ; Enzyme Inhibitors/pharmacology ; Eye Proteins/genetics ; Eye Proteins/metabolism ; Female ; Humans ; Intercellular Signaling Peptides and Proteins/genetics ; Intercellular Signaling Peptides and Proteins/metabolism ; Jurkat Cells ; Leukemia/metabolism ; Male ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; Middle Aged ; Promoter Regions, Genetic ; Proto-Oncogene Proteins/genetics ; Proto-Oncogene Proteins/metabolism ; RNA, Messenger/metabolism ; Wnt Signaling Pathway/genetics ; Young Adult ; DNA Methyltransferase 3B |
| مستخلص: | Background: DNA methylation of CpG islands within the promoters of specific genes may play roles in tumor initiation and progression. It has been suggested such events may serve as critical check points. Methods: The present study analyzed the methylation status of CpG islands within the promoters of secreted frizzled-related proteins (SFRPs) in 87 acute leukemia (AL) patients, 20 normal controls, and four AL cell lines. 5-aza-2'- deoxycytidine (5-Aza-CdR), an inhibitor of DNA methylation, was employed to determine its effect on SFRP expression. Result: Methylation of at least one SFRP promoter was observed in 69% of the AL patients analyzed. In addition, methylation of all four SFRP promoters was observed in Molt-4, Jurkat, HL60 and NB4 cells. In Jurkat cells, methylation levels of four SFRP promoters decreased in a dose-dependent manner upon treatment with 5-Aza-CdR, which coincided with increased mRNA expression. With increasing 5-Aza-CdR concentrations, the expression of DNA methyltransferases, DNMT3A and DNMT3B, significantly decreased in a dose-dependent manner. Conclusion: The present study demonstrated that SFRP gene methylation may be involved in AL progression, with a possible epigenetic mechanism influencing Wnt signaling. |
| المشرفين على المادة: | 0 (Adaptor Proteins, Signal Transducing) 0 (DNMT3A protein, human) 0 (Enzyme Inhibitors) 0 (Eye Proteins) 0 (Frizzled Receptors) 0 (Intercellular Signaling Peptides and Proteins) 0 (Membrane Proteins) 0 (Proto-Oncogene Proteins) 0 (RNA, Messenger) 0 (SFRP1 protein, human) 0 (SFRP2 protein, human) 0 (SFRP4 protein, human) 0 (SFRP5 protein, human) 776B62CQ27 (Decitabine) EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases) EC 2.1.1.37 (DNA Methyltransferase 3A) M801H13NRU (Azacitidine) |
| تواريخ الأحداث: | Date Created: 20120211 Date Completed: 20120628 Latest Revision: 20231213 |
| رمز التحديث: | 20231215 |
| PMID: | 22320963 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2476-762X |
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