دورية أكاديمية
Keratin 8 phosphorylation regulates keratin reorganization and migration of epithelial tumor cells.
| العنوان: | Keratin 8 phosphorylation regulates keratin reorganization and migration of epithelial tumor cells. |
|---|---|
| المؤلفون: | Busch T; Department of Internal Medicine I, University of Ulm, Ulm, Germany., Armacki M, Eiseler T, Joodi G, Temme C, Jansen J, von Wichert G, Omary MB, Spatz J, Seufferlein T |
| المصدر: | Journal of cell science [J Cell Sci] 2012 May 01; Vol. 125 (Pt 9), pp. 2148-59. Date of Electronic Publication: 2012 Feb 17. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Company of Biologists Country of Publication: England NLM ID: 0052457 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-9137 (Electronic) Linking ISSN: 00219533 NLM ISO Abbreviation: J Cell Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge : Company of Biologists Original Publication: London. |
| مواضيع طبية MeSH: | Cell Movement/*drug effects , Cytoskeleton/*metabolism , Epithelial Cells/*metabolism , Intermediate Filaments/*metabolism , Keratin-8/*metabolism, Cell Line, Tumor ; Cell Movement/genetics ; Cytoskeleton/drug effects ; Cytoskeleton/genetics ; Epithelial Cells/drug effects ; Epithelial Cells/pathology ; Extracellular Signal-Regulated MAP Kinases/genetics ; Extracellular Signal-Regulated MAP Kinases/metabolism ; Humans ; Intermediate Filaments/drug effects ; Intermediate Filaments/genetics ; Keratin-8/genetics ; MAP Kinase Kinase Kinases/genetics ; MAP Kinase Kinase Kinases/metabolism ; Pancreatic Neoplasms/metabolism ; Pancreatic Neoplasms/pathology ; Phosphorylation/drug effects ; Phosphorylcholine/analogs & derivatives ; Phosphorylcholine/pharmacology ; Protein Kinase Inhibitors/pharmacology ; Serine/metabolism ; Signal Transduction/drug effects ; Sphingosine/analogs & derivatives ; Sphingosine/pharmacology ; Stomach Neoplasms/metabolism ; Stomach Neoplasms/pathology |
| مستخلص: | Cell migration and invasion are largely dependent on the complex organization of the various cytoskeletal components. Whereas the role of actin filaments and microtubules in cell motility is well established, the role of intermediate filaments in this process is incompletely understood. Organization and structure of the keratin cytoskeleton, which consists of heteropolymers of at least one type 1 and one type 2 intermediate filament, are in part regulated by post-translational modifications. In particular, phosphorylation events influence the properties of the keratin network. Sphingosylphosphorylcholine (SPC) is a bioactive lipid with the exceptional ability to change the organization of the keratin cytoskeleton, leading to reorganization of keratin filaments, increased elasticity, and subsequently increased migration of epithelial tumor cells. Here we investigate the signaling pathways that mediate SPC-induced keratin reorganization and the role of keratin phosphorylation in this process. We establish that the MEK-ERK signaling cascade regulates both SPC-induced keratin phosphorylation and reorganization in human pancreatic and gastric cancer cells and identify Ser431 in keratin 8 as the crucial residue whose phosphorylation is required and sufficient to induce keratin reorganization and consequently enhanced migration of human epithelial tumor cells. |
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| معلومات مُعتمدة: | R01 DK047918 United States DK NIDDK NIH HHS; DK47918 United States DK NIDDK NIH HHS |
| المشرفين على المادة: | 0 (Keratin-8) 0 (Protein Kinase Inhibitors) 10216-23-6 (sphingosine phosphorylcholine) 107-73-3 (Phosphorylcholine) 452VLY9402 (Serine) EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases) EC 2.7.11.25 (MAP Kinase Kinase Kinases) NGZ37HRE42 (Sphingosine) |
| تواريخ الأحداث: | Date Created: 20120221 Date Completed: 20121023 Latest Revision: 20211021 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC3367938 |
| DOI: | 10.1242/jcs.080127 |
| PMID: | 22344252 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1477-9137 |
|---|---|
| DOI: | 10.1242/jcs.080127 |