دورية أكاديمية
أعرض في EDS

Dietary long-chain polyunsaturated fatty acids upregulate expression of FADS3 transcripts.

التفاصيل البيبلوغرافية
العنوان: Dietary long-chain polyunsaturated fatty acids upregulate expression of FADS3 transcripts.
المؤلفون: Reardon HT; Division of Nutritional Sciences, Cornell University, Ithaca, New York, USA., Hsieh AT, Park WJ, Kothapalli KS, Anthony JC, Nathanielsz PW, Brenna JT
المصدر: Prostaglandins, leukotrienes, and essential fatty acids [Prostaglandins Leukot Essent Fatty Acids] 2013 Jan; Vol. 88 (1), pp. 15-9. Date of Electronic Publication: 2012 Mar 06.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Churchill Livingstone Country of Publication: Scotland NLM ID: 8802730 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2823 (Electronic) Linking ISSN: 09523278 NLM ISO Abbreviation: Prostaglandins Leukot Essent Fatty Acids Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Edinburgh ; New York : Churchill Livingstone, c1988-
مواضيع طبية MeSH: Gene Expression Regulation, Enzymologic*/drug effects, Arachidonic Acids/*metabolism , Dietary Fats/*administration & dosage , Docosahexaenoic Acids/*administration & dosage , Fatty Acid Desaturases/*biosynthesis , Liver/*metabolism, Alternative Splicing/drug effects ; Animals ; Animals, Newborn ; Arachidonic Acids/deficiency ; Delta-5 Fatty Acid Desaturase ; Dietary Fats/metabolism ; Docosahexaenoic Acids/metabolism ; Down-Regulation/drug effects ; Enzyme Induction/drug effects ; Fatty Acid Desaturases/genetics ; Fatty Acid Desaturases/metabolism ; Female ; Hep G2 Cells ; Humans ; Isoenzymes/biosynthesis ; Isoenzymes/genetics ; Isoenzymes/metabolism ; Liver/drug effects ; Liver/growth & development ; Male ; PPAR gamma/antagonists & inhibitors ; PPAR gamma/metabolism ; Papio ; RNA, Messenger/metabolism ; Random Allocation
مستخلص: The fatty acid desaturase (FADS) gene family at 11q12-13.1 includes FADS1 and FADS2, both known to mediate biosynthesis of omega-3 and omega-6 long-chain polyunsaturated fatty acids (LCPUFA). FADS3 is a putative desaturase due to its sequence similarity with FADS1 and FADS2, but its function is unknown. We have previously described 7 FADS3 alternative transcripts (AT) and 1 FADS2 AT conserved across multiple species. This study examined the effect of dietary LCPUFA levels on liver FADS gene expression in vivo and in vitro, evaluated by qRT-PCR. Fourteen baboon neonates were randomized to three diet groups for their first 12 weeks of life, C: Control, no LCPUFA, L: 0.33% docosahexaenoic acid (DHA)/0.67% arachidonic acid (ARA) (w/w); and L3: 1.00% DHA/0.67% ARA (w/w). Liver FADS1 and both FADS2 transcripts were downregulated by at least 50% in the L3 group compared to controls. In contrast, FADS3 AT were upregulated (L3 > C), with four transcripts significantly upregulated by 40% or more. However, there was no evidence for a shift in liver fatty acids to coincide with increased FADS3 expression. Significant upregulation of FADS3 AT was also observed in human liver-derived HepG2 cells after DHA or ARA treatment. The PPARγ antagonist GW9662 prevented FADS3 upregulation, while downregulation of FADS1 and FADS2 was unaffected. Thus, FADS3 AT were directly upregulated by LCPUFA by a PPARγ-dependent mechanism unrelated to regulation of other desaturases. This opposing pattern and mechanism of regulation suggests a dissimilar function for FADS3 AT compared to other FADS gene products.
(Copyright © 2012 Elsevier Ltd. All rights reserved.)
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معلومات مُعتمدة: T32 DK007158 United States DK NIDDK NIH HHS; T32 HD052471 United States HD NICHD NIH HHS; P51 OD011133 United States OD NIH HHS; T32HD052471 United States HD NICHD NIH HHS; T32 HD052471-05 United States HD NICHD NIH HHS; T32 DK007158-33 United States DK NIDDK NIH HHS
المشرفين على المادة: 0 (Arachidonic Acids)
0 (Delta-5 Fatty Acid Desaturase)
0 (Dietary Fats)
0 (Isoenzymes)
0 (PPAR gamma)
0 (RNA, Messenger)
25167-62-8 (Docosahexaenoic Acids)
EC 1.14.19.- (Fatty Acid Desaturases)
EC 1.14.19.3 (FADS1 protein, human)
EC 1.14.19.3 (FADS3 protein, human)
تواريخ الأحداث: Date Created: 20120309 Date Completed: 20130703 Latest Revision: 20211203
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC3386357
DOI: 10.1016/j.plefa.2012.02.003
PMID: 22398025
قاعدة البيانات: MEDLINE
الوصف
تدمد:1532-2823
DOI:10.1016/j.plefa.2012.02.003