التفاصيل البيبلوغرافية
| العنوان: |
Noninvasive method for monitoring ethanol in fermentation processes using fiber-optic near-infrared spectroscopy. |
| المؤلفون: |
Cavinato AG; Department of Chemistry, University of Washington, Seattle 98195., Mayes DM, Ge ZH, Callis JB |
| المصدر: |
Analytical chemistry [Anal Chem] 1990 Sep 15; Vol. 62 (18), pp. 1977-82. |
| نوع المنشور: |
Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print Cited Medium: Print ISSN: 0003-2700 (Print) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Washington, American Chemical Society. |
| مواضيع طبية MeSH: |
Ethanol/*analysis, Fermentation ; Fiber Optic Technology ; Optical Fibers ; Scattering, Radiation ; Spectrophotometry, Infrared |
| مستخلص: |
Short-wavelength near-infrared (SW-near-IR) spectroscopy (700-1100 nm) is used for the determination of ethanol during the time course of a fermentation. Measurements are performed noninvasively by means of a photodiode array spectrometer equipped with a fiber-optic probe placed on the outside of the glass-wall fermentation vessel. Pure ethanol/water and ethanol/yeast/water mixtures are studied to establish the spectral features that characterize ethanol and to show that determination of ethanol is independent of the yeast concentration. Analysis of the second-derivative data is accomplished with multilinear regression (MLR). The standard error of prediction (SEP) of ethanol in ethanol/water solutions is approximately 0.2% over a range of 0-15%; the SEP of ethanol in ethanol/yeast/water solutions is 0.27% (w/w). Results from the mixture experiments are then applied to actual yeast fermentations of glucose to ethanol. By use of a gas chromatographic method for validation, a good correlation is found between the intensity of backscattered light at 905 nm and the actual ethanol. Additional experiments show that a calibration model created for one fermentation can be used to predict ethanol production during the time course of others with a prediction error of 0.4%. |
| المشرفين على المادة: |
3K9958V90M (Ethanol) |
| تواريخ الأحداث: |
Date Created: 19900915 Date Completed: 19901206 Latest Revision: 20190717 |
| رمز التحديث: |
20221208 |
| DOI: |
10.1021/ac00217a015 |
| PMID: |
2240577 |
| قاعدة البيانات: |
MEDLINE |
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