دورية أكاديمية
The effects of nanofiber topography on astrocyte behavior and gene silencing efficiency.
| العنوان: | The effects of nanofiber topography on astrocyte behavior and gene silencing efficiency. |
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| المؤلفون: | Cao H; School of Chemical and Biomedical Engineering, Nanyang Technological University, 637459 Singapore., Marcy G, Goh EL, Wang F, Wang J, Chew SY |
| المصدر: | Macromolecular bioscience [Macromol Biosci] 2012 May; Vol. 12 (5), pp. 666-74. Date of Electronic Publication: 2012 Mar 13. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101135941 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1616-5195 (Electronic) Linking ISSN: 16165187 NLM ISO Abbreviation: Macromol Biosci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Weinheim, Germany : Wiley-VCH, c2001- |
| مواضيع طبية MeSH: | Nerve Regeneration*, Astrocytes/*drug effects , Nanofibers/*chemistry , Polyesters/*chemical synthesis , Tissue Engineering/*methods, Animals ; Apoptosis/drug effects ; Astrocytes/cytology ; Astrocytes/physiology ; Cell Proliferation/drug effects ; Cerebral Cortex/cytology ; Cerebral Cortex/drug effects ; Cerebral Cortex/physiology ; Electrochemical Techniques ; Embryo, Mammalian ; Endocytosis/drug effects ; Gene Silencing ; Microscopy, Electron, Scanning ; Nanofibers/ultrastructure ; Polyesters/pharmacology ; Primary Cell Culture ; RNA, Small Interfering/genetics ; Rats ; Tissue Scaffolds ; Transfection |
| مستخلص: | Astrocyte-nanofiber interactions are studied by culturing primary rat cortical astrocytes on poly[caprolactone-co-(ethyl ethylene phosphate)] electrospun nanofibers and solvent-cast films (two-dimensional control). The results indicate that nanofiber topography significantly suppresses astrocyte proliferation and enhances apoptosis, without altering cellular activation as compared to films. Moreover, nanofiber topography enhances gene-silencing efficiency in astrocytes. The results suggest that nanofibers may serve as potential substrates for nerve regeneration by suppressing astrocyte growth and may further facilitate the use of gene-silencing to enhance CNS regeneration. (Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.) |
| المشرفين على المادة: | 0 (Polyesters) 0 (RNA, Small Interfering) 0 (poly(epsilon-caprolactone)-block-poly(ethyl ethylene phosphate)) |
| تواريخ الأحداث: | Date Created: 20120314 Date Completed: 20121108 Latest Revision: 20120508 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1002/mabi.201100436 |
| PMID: | 22411782 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1616-5195 |
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| DOI: | 10.1002/mabi.201100436 |