دورية أكاديمية
Mxi1 influences cyst formation in three-dimensional cell culture.
| العنوان: | Mxi1 influences cyst formation in three-dimensional cell culture. |
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| المؤلفون: | Yook YJ; Department of Biological Science, Sookmyung Women's University, Seoul, Korea., Yoo KH, Song SA, Seo MJ, Ko JY, Kim BH, Lee EJ, Chang E, Woo YM, Park JH |
| المصدر: | BMB reports [BMB Rep] 2012 Mar; Vol. 45 (3), pp. 189-93. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Korean Society for Biochemistry and Molecular Biology Country of Publication: Korea (South) NLM ID: 101465334 Publication Model: Print Cited Medium: Internet ISSN: 1976-670X (Electronic) Linking ISSN: 19766696 NLM ISO Abbreviation: BMB Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Seoul : Korean Society for Biochemistry and Molecular Biology |
| مواضيع طبية MeSH: | Basic Helix-Loop-Helix Transcription Factors/*metabolism , Cysts/*metabolism , Tumor Suppressor Proteins/*metabolism, Animals ; Apoptosis ; Basic Helix-Loop-Helix Transcription Factors/genetics ; Cell Culture Techniques ; Cell Survival ; Cells, Cultured ; Mice ; Rats ; Real-Time Polymerase Chain Reaction ; Tumor Suppressor Proteins/genetics |
| مستخلص: | Cyst formation is a major characteristic of ADPKD and is caused by the abnormal proliferation of epithelial cells. Renal cyst formation disrupts renal function and induces diverse complications. The mechanism of cyst formation is unclear. mIMCD-3 cells were established to develop simple epithelial cell cysts in 3-D culture. We confirmed previously that Mxi1 plays a role in cyst formation in Mxi1-deficient mice. Cysts in Mxi1 transfectanted cells were showed by collagen or mebiol gels in 3-D cell culture system. Causative genes of ADPKD were measured by q RT-PCR. Herein, Mxi1 transfectants rarely formed a simple epithelial cyst and induced cell death. Overexpression of Mxi1 resulted in a decrease in the PKD1, PKD2 and c-myc mRNA relating to the pathway of cyst formation. These data indicate that Mxi1 influences cyst formation of mIMCD-3 cells in 3-D culture and that Mxi1 may control the mechanism of renal cyst formation. |
| المشرفين على المادة: | 0 (Basic Helix-Loop-Helix Transcription Factors) 0 (Mxi1 protein, mouse) 0 (Tumor Suppressor Proteins) |
| تواريخ الأحداث: | Date Created: 20120328 Date Completed: 20141222 Latest Revision: 20181023 |
| رمز التحديث: | 20240628 |
| DOI: | 10.5483/BMBRep.2012.45.3.189 |
| PMID: | 22449707 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1976-670X |
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| DOI: | 10.5483/BMBRep.2012.45.3.189 |